CD134 Recombinant Rabbit Monoclonal Antibody [JE36-48]
Catalog# HA722700
CD134 Recombinant Rabbit Monoclonal Antibody [JE36-48]
-
WB
-
Human
-
unconjugated
Overview
Product Name
CD134 Recombinant Rabbit Monoclonal Antibody [JE36-48]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CD134 aa 1-250 / 277.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 29 kDa
Positive Control
HUT 102 cell lysate.
Conjugation
unconjugated
Clone Number
JE36-48
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor, is a member of the TNFR-superfamily of receptors which is not constitutively expressed on resting naïve T cells, unlike CD28. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation. Expression of OX40 is dependent on full activation of the T cell; without CD28, expression of OX40 is delayed and of fourfold lower levels.
Background References
1. Kwiatek M et al. OX40 (CD134) Expression on T Regulatory Cells Is Related to Serious Hypertensive Disorders in Pregnancy. J Cardiovasc Dev Dis. 2023 Oct
2. Ansari AW et al. Azithromycin downregulates ICOS (CD278) and OX40 (CD134) expression and mTOR activity of TCR-activated T cells to inhibit proliferation. Int Immunopharmacol. 2023 Nov
Subcellular Location
Membrane.
UNIPROT
Synonyms
ACT 35 antibody
ACT35 antibody
ACT35 antigen antibody
ATC35 antigen antibody
CD 134 antibody
CD134 antibody
CD134 antigen antibody
IMD16 antibody
Lymphoid activation antigene ACT35 antibody
OX 40 antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CD134 on different lysates with Rabbit anti-CD134 antibody (HA722700) at 1/1,000 dilution.
Lane 1: HUT 102 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 50 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CD134 Recombinant Rabbit Monoclonal Antibody [PSH10-14] - BSA and Azide free
Application: WB,IF-Cell,FC,IP
Reactivity: Human
Conjugate: unconjugated
CD134 Recombinant Rabbit Monoclonal Antibody [PSH10-14]
Application: WB,IF-Cell,FC,IP
Reactivity: Human
Conjugate: unconjugated
