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CD23 Recombinant Rabbit Monoclonal Antibody [PD00-03]

Usd: 385

Specification

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Catalog# HA721139

CD23 Recombinant Rabbit Monoclonal Antibody [PD00-03]

Application

  • IHC-P

  • mIHC

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD23 Recombinant Rabbit Monoclonal Antibody [PD00-03]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human CD23 aa 48 – 321 (Extracellular).

Species Reactivity

Human

Validated Applications

IHC-P, mIHC, IF-Tissue

Molecular Weight

Predicted band size: 36 kDa

Positive Control

Human tonsil tissue, human lymph nodes tissue, human spleen tissue.

Conjugation

unconjugated

Clone Number

PD00-03

RRID

AB_3072261

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:200-1:2,000

  • mIHC

  • 1:800-1:4,000

  • IF-Tissue

  • 1:200

Target

Function

CD23 (low affinity IgE receptor, Leu-20, FceRII) is a type II integral membrane glycoprotein, 45-60 kDa, and a member of an immunoglobulin supergene family. CD23 is a B-cell-specific antigen and it has essential roles in the regulation of IgE production and in the differentiation of B-cells. In humans, main cellular expression of CD23 is found in B-lymphocytes (strong expression in activated germinal center B-cells, weaker staining of resting mantle zone B-cells), monocytes, follicular dendritic cells (FDCs) predominately in the apical light zone of the germinal center, but also on activated CD4+ T lymphocyte subset, platelets, eosinophils, neutrophils, and Langerhans cells. CD23 is upregulated in Eppstein-Barr infection. CD23 is constitutively expressed by intestinal epithelial cells and its expression is enhanced in enteropathies. CD23 is typically expressed in chronic lymphocytic leukemia (CLL), sometimes in follicular lymphoma, rarely in marginal zone and lymphoplasmacytic lymphoma, but not in mantle cell lymphoma. In CLL, the strongest expression is characteristically present in proliferation centers. It was recently demonstrated that patients with CD23-positive diffuse large B-cell lymphoma have good prognosis. CD23 is also frequently used to demonstrate benign follicular dendritic cells in the background of follicular lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, (intra)follicular T-cell lymphoma and other lymphomas. Together with CD21, CD23 is a marker of rare follicular dendritic cell tumors Some authors demonstrated CD23 expression in epithelial cells of nasopharyngeal carcinoma. In diagnostic pathology CD23 is primarily used in the panel for small B-cell lymphoproliferative disorders. Tonsil is recommended as positive and negative tissue control for CD23. The follicular dendritic cells of the germinal centres must be stained as strongly as possible without any staining reaction of squamous epithelial cells and T-cells in the interfollicular T-zones. In the mantle zone of the follicles, the majority of activated B-cells must show an at least weak to moderate and distinct continuous membranous staining reaction.

Background References

1. Engeroff P. et. al. The role of CD23 in the regulation of allergic responses. Allergy. 2021 Jul

2. Engeroff P. et. al. CD23 provides a noninflammatory pathway for IgE-allergen complexes. J Allergy Clin Immunol. 2020 Jan

Subcellular Location

Cell membrane, Secreted.

UNIPROT

SwissProt: P06734 Human

Synonyms

Blast 2 antibody

BLAST-2 antibody

Blast2 antibody

C type lectin domain family 4 member J antibody

C-type lectin domain family 4 antibody

C-type lectin domain family 4 member J antibody

C-type lectin domain family 4, member J antibody

CD 23 antibody

CD 23A antibody

CD23 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD23 antibody (<a href="/products/HA721139" style="font-weight: bold;text-decoration: underline;">HA721139</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721139" style="font-weight: bold;text-decoration: underline;">HA721139</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD23 antibody (HA721139) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721139) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD23 antibody (<a href="/products/HA721139" style="font-weight: bold;text-decoration: underline;">HA721139</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721139" style="font-weight: bold;text-decoration: underline;">HA721139</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD23 antibody (HA721139) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721139) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD23 antibody (<a href="/products/HA721139" style="font-weight: bold;text-decoration: underline;">HA721139</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721139" style="font-weight: bold;text-decoration: underline;">HA721139</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD23 antibody (HA721139) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721139) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Fluorescence multiplex immunohistochemical analysis of Human tonsil (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD68 (<a href="/products/HA601115" style="font-weight: bold;text-decoration: underline;">HA601115</a>, Red), anti-CD38 (<a href="/products/HA721268" style="font-weight: bold;text-decoration: underline;">HA721268</a>, Green), anti-CD23 (<a href="/products/HA721139" style="font-weight: bold;text-decoration: underline;">HA721139</a>, White), anti-CD11C (<a href="/products/ET1606-19" style="font-weight: bold;text-decoration: underline;">ET1606-19</a>, Cyan), anti-CD45 (<a href="/products/ET7111-03" style="font-weight: bold;text-decoration: underline;">ET7111-03</a>, Magenta) and anti-CD20 (<a href="/products/HA721138" style="font-weight: bold;text-decoration: underline;">HA721138</a>, Yellow) on tonsil. Panel B: anti-CD68 stained on Macrophage. Panel C: anti-CD38 stained on lymphocyte subsets. Panel D: anti-CD11C stained on dendritic cells. Panel E: CD45 stained on lymphocytes. Panel F: anti-CD20 stained on B cells. Panel G: anti-CD23 stained on follicular dendritic cells. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit&trade;MH010101, www.luminiris.cn). The section was incubated in six rounds of staining: in the order of <a href="/products/HA601115" style="font-weight: bold;text-decoration: underline;">HA601115</a> (1/2,000 dilution), <a href="/products/HA721268" style="font-weight: bold;text-decoration: underline;">HA721268</a> (1/1,000 dilution), <a href="/products/ET1606-19" style="font-weight: bold;text-decoration: underline;">ET1606-19</a> (1/1,000 dilution), <a href="/products/ET7111-03" style="font-weight: bold;text-decoration: underline;">ET7111-03</a> (1/500 dilution), <a href="/products/HA721138" style="font-weight: bold;text-decoration: underline;">HA721138</a> (1/2,000 dilution) and <a href="/products/HA721139" style="font-weight: bold;text-decoration: underline;">HA721139</a> (1/800 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    Fluorescence multiplex immunohistochemical analysis of Human tonsil (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD68 (HA601115, Red), anti-CD38 (HA721268, Green), anti-CD23 (HA721139, White), anti-CD11C (ET1606-19, Cyan), anti-CD45 (ET7111-03, Magenta) and anti-CD20 (HA721138, Yellow) on tonsil. Panel B: anti-CD68 stained on Macrophage. Panel C: anti-CD38 stained on lymphocyte subsets. Panel D: anti-CD11C stained on dendritic cells. Panel E: CD45 stained on lymphocytes. Panel F: anti-CD20 stained on B cells. Panel G: anti-CD23 stained on follicular dendritic cells. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in six rounds of staining: in the order of HA601115 (1/2,000 dilution), HA721268 (1/1,000 dilution), ET1606-19 (1/1,000 dilution), ET7111-03 (1/500 dilution), HA721138 (1/2,000 dilution) and HA721139 (1/800 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: tonsil<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Human

    Site: tonsil

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: lymph nodes<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Human

    Site: lymph nodes

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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