CD23 Recombinant Antibody [PD00-03] - Mouse IgG1 (Chimeric)
Catalog# HA601682
CD23 Recombinant Antibody [PD00-03] - Mouse IgG1 (Chimeric)
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
CD23 Recombinant Antibody [PD00-03] - Mouse IgG1 (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Immunogen
Recombinant protein within human CD23 aa 48 – 321 (Extracellular).
Species Reactivity
Human
Validated Applications
IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 36 kDa
Positive Control
Human Tonsil, Human Lymph node.
Conjugation
unconjugated
Clone Number
PD00-03
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| IHC-P | IF-Tissue | |
|---|---|---|
| human |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:1,000
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IF-Tissue
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1:200
Target
Function
CD23 (low affinity IgE receptor, Leu-20, FceRII) is a type II integral membrane glycoprotein, 45-60 kDa, and a member of an immunoglobulin supergene family. CD23 is a B-cell-specific antigen and it has essential roles in the regulation of IgE production and in the differentiation of B-cells. In humans, main cellular expression of CD23 is found in B-lymphocytes (strong expression in activated germinal center B-cells, weaker staining of resting mantle zone B-cells), monocytes, follicular dendritic cells (FDCs) predominately in the apical light zone of the germinal center, but also on activated CD4+ T lymphocyte subset, platelets, eosinophils, neutrophils, and Langerhans cells. CD23 is upregulated in Eppstein-Barr infection. CD23 is constitutively expressed by intestinal epithelial cells and its expression is enhanced in enteropathies. CD23 is typically expressed in chronic lymphocytic leukemia (CLL), sometimes in follicular lymphoma, rarely in marginal zone and lymphoplasmacytic lymphoma, but not in mantle cell lymphoma. In CLL, the strongest expression is characteristically present in proliferation centers. It was recently demonstrated that patients with CD23-positive diffuse large B-cell lymphoma have good prognosis. CD23 is also frequently used to demonstrate benign follicular dendritic cells in the background of follicular lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, (intra)follicular T-cell lymphoma and other lymphomas. Together with CD21, CD23 is a marker of rare follicular dendritic cell tumors Some authors demonstrated CD23 expression in epithelial cells of nasopharyngeal carcinoma. In diagnostic pathology CD23 is primarily used in the panel for small B-cell lymphoproliferative disorders. Tonsil is recommended as positive and negative tissue control for CD23. The follicular dendritic cells of the germinal centres must be stained as strongly as possible without any staining reaction of squamous epithelial cells and T-cells in the interfollicular T-zones. In the mantle zone of the follicles, the majority of activated B-cells must show an at least weak to moderate and distinct continuous membranous staining reaction.
Background References
1. Engeroff P. et. al. The role of CD23 in the regulation of allergic responses. Allergy. 2021 Jul
2. Engeroff P. et. al. CD23 provides a noninflammatory pathway for IgE-allergen complexes. J Allergy Clin Immunol. 2020 Jan
Subcellular Location
Cell membrane, Secreted.
UNIPROT
Synonyms
Blast 2 antibody
BLAST-2 antibody
Blast2 antibody
C type lectin domain family 4 member J antibody
C-type lectin domain family 4 antibody
C-type lectin domain family 4 member J antibody
C-type lectin domain family 4, member J antibody
CD 23 antibody
CD 23A antibody
CD23 antibody
ExpandImages
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Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue:Tonsil
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601682, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Lymph node
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601682, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1120, 20 minutes at room temperature. -
Application: Immunofluorescence (IF-tissue)
Species: Human
Tissue: Tonsil
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601682, 1/200, overnight at 4°C.
Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CD23 Rabbit Polyclonal Antibody
Application: IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
CD23 Recombinant Rabbit Monoclonal Antibody
Application: mIHC
Reactivity: Human
Conjugate: unconjugated
