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HRP Conjugated Goat anti-Mouse IgG UltraPolymer Antibody

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Catalog# HA1120

HRP Conjugated Goat anti-Mouse IgG UltraPolymer Antibody

Application

  • IHC-P

Reactivity

  • Mouse

Conjugation

  • HRP

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HRP Conjugated Goat anti-Mouse IgG UltraPolymer Antibody

Antibody Type

Goat Polyclonal Antibody

Immunogen

Purified Mouse IgG, whole molecule

Species Reactivity

Mouse

Validated Applications

IHC-P

Conjugation

HRP

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at 2-8 ℃.

Storage Buffer

TBS, 1% (w/v) BSA, 0.1% Proclin 150, 10% heat-inactivated animal serum.

Isotype

IgG

Purification Method

Affinity purified using solid phase Mouse IgG.

Application Dilution

  • IHC-P

  • Ready to Use

Target

Function

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells. UltraPolymer Goat anti-Mouse IgG (H&L) conjugated to HRP, affinity purified, min x w/ bovine, horse, human, pig or rabbit serum proteins.

Background References

暂无

Images

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MSH2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The detection was performed using an Goat anti-Mouse IgG (H&L) - HRP (<a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>). Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MSH2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The detection was performed using an Goat anti-Mouse IgG (H&L) - HRP (HA1120). Counter stained with hematoxylin.

  • Analysis of HeLa cells labeling p16 with Mouse anti-p16 antibody (<a href="/products/HA601131" style="font-weight: bold;text-decoration: underline;">HA601131</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16 antibody (<a href="/products/HA601131" style="font-weight: bold;text-decoration: underline;">HA601131</a>) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. The detection was performed using an Goat anti-Mouse IgG (H&L) - HRP (<a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>).

    Analysis of HeLa cells labeling p16 with Mouse anti-p16 antibody (HA601131) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p16 antibody (HA601131) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. The detection was performed using an Goat anti-Mouse IgG (H&L) - HRP (HA1120).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation