CD3 epsilon Recombinant Rabbit Monoclonal Antibody [JE17-74]
Catalog# ET7110-63
CD3 epsilon Recombinant Rabbit Monoclonal Antibody [JE17-74]
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WB
-
IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
CD3 epsilon Recombinant Rabbit Monoclonal Antibody [JE17-74]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CD3 epsilon aa 158-207 / 207.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Molecular Weight
23 kDa
Positive Control
Jurkat cell lysates, human tonsil tissue, human spleen tissue, Jurkat.
Conjugation
unconjugated
Clone Number
JE17-74
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.
Background References
1. Chen L. et. al. The dynamic changes of CD3e-CD11c+ dendritic cells in spleens and bone marrow of mice infected with Schistosoma japonicum. Parasitol Res. 2017 Mar;116(3):1007-1011.
2. Firtina S. et. al. A novel pathogenic frameshift variant of CD3E gene in two T-B+ NK+ SCID patients from Turkey. Immunogenetics. 2017 Oct;69(10):653-659.
Post-translational Modification
Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD3 epsilon antibody
CD3e antibody
CD3e antigen antibody
CD3e antigen epsilon polypeptide (TiT3 complex) antibody
CD3E antigen epsilon polypeptide antibody
CD3E antigen
epsilon subunit antibody
CD3e molecule epsilon antibody
CD3e molecule
epsilon (CD3 TCR complex) antibody
ExpandImages
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Western blot analysis of CD3 epsilon on Jurkat cell lysates with Rabbit anti-CD3 epsilon antibody (ET7110-63) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 23 kDa
Observed band size:23 kDa
Exposure time: 2 minutes;
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-63) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD3 epsilon antibody (ET7110-63) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-63) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD3 epsilon antibody (ET7110-63) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-63) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of CD3 epsilon was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-63, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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