CD3 zeta Recombinant Rabbit Monoclonal Antibody [JE09-37]
Catalog# HA722692
CD3 zeta Recombinant Rabbit Monoclonal Antibody [JE09-37]
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WB
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IHC-P
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IF-Tissue
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Human
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Rat
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unconjugated
Overview
Product Name
CD3 zeta Recombinant Rabbit Monoclonal Antibody [JE09-37]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human CD3 zeta aa 115-164 / 164.
Species Reactivity
Human, Rat
Validated Applications
WB, IHC-P, IF-Tissue
Target Molecular Weight
Predicted band size: 19 kDa
Positive Control
Jurkat cell lysate, human spleen tissue, human thymus tissue, human tonsil tissue, rat spleen tissue.
Conjugation
unconjugated
Clone Number
JE09-37
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
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| human |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
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IF-Tissue
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1:200
Target
Function
T-cell surface glycoprotein CD3 zeta chain also known as T-cell receptor T3 zeta chain or CD247 (Cluster of Differentiation 247) is a protein that in humans is encoded by the CD247 gene. Some older literature mention a similar protein called "CD3 eta" in mice. It is now understood to be an isoform differing in the last exon. T-cell receptor zeta (ζ), together with T-cell receptor alpha/beta and gamma/delta heterodimers and CD3-gamma, -delta, and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Background References
1. Velasco Cárdenas RM et al. Harnessing CD3 diversity to optimize CAR T cells. Nat Immunol. 2023 Dec
2. Kath J et al. Integration of zeta-deficient CARs into the CD3-zeta gene conveys potent cytotoxicity in T and NK cells. Blood. 2024 Mar
Subcellular Location
Cell membrane.
Synonyms
4930549J05Rik antibody
A430104F18Rik antibody
AW552088 antibody
CD247 antibody
CD247 antigen antibody
Cd247 molecule antibody
Cd3 antibody
CD3 antigen, zeta polypeptide, isoform CRA_b antibody
CD3 antigen, zeta subunit antibody
CD3-eta antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CD3 zeta on different lysates with Rabbit anti-CD3 zeta antibody (HA722692) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: 293T cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 19 kDa
Observed band size: 16 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722692) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD3 zeta antibody (HA722692) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722692) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-CD3 zeta antibody (HA722692) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722692) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD3 zeta antibody (HA722692) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722692) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD3 zeta antibody (HA722692) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722692) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IF-Tissue
Species: Human
Site: Spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/200
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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