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CD38 Recombinant Rabbit Monoclonal Antibody [PSH20-93]

Usd: 385

Specification

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Catalog# HA724188

CD38 Recombinant Rabbit Monoclonal Antibody [PSH20-93]

Application

  • IF-Cell

  • FC

Reactivity

  • Mouse

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

CD38 Recombinant Rabbit Monoclonal Antibody [PSH20-93]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse CD38 aa 1-304.

Species Reactivity

Mouse

Validated Applications

IF-Cell, FC

Target Molecular Weight

Predicted band size: 34 kDa

Positive Control

Mouse splenocytes.

Conjugation

unconjugated

Clone Number

PSH20-93

Reactivity Data

IF-Cell FC
Mouse
Tested Tested
Tested Tested
Human
Rat

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 1% BSA, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Cell

  • 1:500

  • FC

  • 1:1,000

Target

Function

CD38 is a type II integral membrane glycoprotein which is present on early B and T cell lineages and activated B and T cells but is absent from most mature resting peripheral lymphocytes. CD38 is also found on thymocytes, pre-B cells, germinal center B cells, mitogen-activated T cells, monocytes and Ig-secreting plasma cells. CD38 acts as a NAD glycohydrolase in T lymphocytes. On hematopoietic cells CD38 induces activation, proliferation, and differentiation of mature T and B cells and mediates apoptosis of myeloid and lymphoid progenitor cells. In addition to acting as a signaling receptor, CD38 is also an enzyme capable of producing several calcium-mobilizing metabolites, including cyclic adenosine diphosphate ribose (cADPR). CD38 also plays a role in maintaining survival of an invariant NK T (iNKT) cell subset that preferentially contributes to the maintenance of immunological tolerance.

Background References

1. Yang Q et al. NADase CD38 is a key determinant of ovarian aging. Nat Aging. 2024 Jan

2. Yu S et al. CD38-Targeting Peptide Vaccine Ameliorates Aging-Associated Phenotypes in Mice. Aging Cell. 2025 Sep

Subcellular Location

Membrane.

UNIPROT

SwissProt: P56528 Mouse

Synonyms

Acute lymphoblastic leukemia cells antigen CD38 antibody

ADP ribosyl cyclase 1 antibody

ADP ribosyl cyclase antibody

ADP ribosyl cyclase/cyclic ADP-ribose hydrolase antibody

ADP-ribosyl cyclase 1 antibody

ADPRC 1 antibody

ADPRC1 antibody

cADPr hydrolase 1 antibody

CD 38 antibody

CD38 antibody

Expand

Images

  • Immunocytochemistry analysis of mouse splenocytes labeling CD38 with Rabbit anti-CD38 antibody (<a href="/products/HA724188" style="font-weight: bold;text-decoration: underline;">HA724188</a>) at 1/500 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD38 antibody (<a href="/products/HA724188" style="font-weight: bold;text-decoration: underline;">HA724188</a>) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of mouse splenocytes labeling CD38 with Rabbit anti-CD38 antibody (HA724188) at 1/500 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD38 antibody (HA724188) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of mouse splenocytes labeling CD38.<br /><br />Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (<a href="/products/HA724188" style="font-weight: bold;text-decoration: underline;">HA724188</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of mouse splenocytes labeling CD38.

    Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA724188, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"