CD40L Recombinant Rabbit Monoclonal Antibody [JM11-34] - BSA and Azide free
Catalog# HA750387
CD40L Recombinant Rabbit Monoclonal Antibody [JM11-34] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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ET1703-64
含抗保成分
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unconjugated
Overview
Product Name
CD40L Recombinant Rabbit Monoclonal Antibody [JM11-34] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CD40L aa 103-150 / 261.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 29 kDa
Positive Control
Jurkat cell lysate, K-562 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, rat thymus tissue lysate.
Conjugation
unconjugated
Clone Number
JM11-34
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
Target
Function
Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. While cytokines IL-4 and IL-13 alone are inadequate for B cell activation, contact with Th cells seems to be sufficient for delivery of proliferative signals. CD40 and CD154 (also designated CD40L or TRAP) comprise a receptor ligand pair central to the transmission of this signal. CD40 is expressed on the surface of B cells and CD154 is expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. CD154 is a 261 amino acid protein that is is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. Its expression is tightly regulated, and abnormal levels of CD154 are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1.
Background References
1. Matus-Nicodemos R et al. Polypyrimidine tract-binding protein is critical for the turnover and subcellular distribution of CD40 ligand mRNA in CD4+ T cells. J Immunol 186:2164-71 (2011).
2. Hwang Y et al. Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L. Infect Immun 68:511-7 (2000).
Sequence Similarity
Belongs to the tumor necrosis factor family.
Tissue Specificity
Specifically expressed on activated CD4+ T-lymphocytes.
Post-translational Modification
The soluble form derives from the membrane form by proteolytic processing.; N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.; Not O-glycosylated.
Subcellular Location
Cell membrane, Cell surface, Secreted.
Synonyms
CD 40L antibody
CD154 antibody
CD40 antigen ligand antibody
CD40 ligand antibody
CD40 ligand, soluble form antibody
CD40-L antibody
CD40L antibody
CD40L_HUMAN antibody
CD40LG antibody
gp39 antibody
ExpandImages
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Western blot analysis of CD40L on different lysates with Rabbit anti-CD40L antibody (HA750387) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate (15 µg/Lane)
Lane 2: K-562 cell lysate (15 µg/Lane)
Lane 3: PC-12 cell lysate (15 µg/Lane)
Lane 4: Mouse liver tissue lysate (20 µg/Lane)
Lane 5: Rat thymus tissue lysate (20 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 40 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750387) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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