CD40L Recombinant Rabbit Monoclonal Antibody [JM11-34]

Function

Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. While cytokines IL-4 and IL-13 alone are inadequate for B cell activation, contact with Th cells seems to be sufficient for delivery of proliferative signals. CD40 and CD154 (also designated CD40L or TRAP) comprise a receptor ligand pair central to the transmission of this signal. CD40 is expressed on the surface of B cells and CD154 is expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. CD154 is a 261 amino acid protein that is is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. Its expression is tightly regulated, and abnormal levels of CD154 are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1.

Background References

1. Matus-Nicodemos R et al. Polypyrimidine tract-binding protein is critical for the turnover and subcellular distribution of CD40 ligand mRNA in CD4+ T cells. J Immunol 186:2164-71 (2011).

2. Hwang Y et al. Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L. Infect Immun 68:511-7 (2000).

Sequence Similarity

Belongs to the tumor necrosis factor family.

Tissue Specificity

Specifically expressed on activated CD4+ T-lymphocytes.

Post-translational Modification

The soluble form derives from the membrane form by proteolytic processing.; N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.; Not O-glycosylated.

Subcellular Location

Cell membrane, Cell surface, Secreted.

UNIPROT

SwissProt: P29965 Human

SwissProt: P27548 Mouse

SwissProt: Q9Z2V2 Rat

Synonyms