CD42b Mouse Monoclonal Antibody [13G2]
Catalog# EM1902-15
CD42b Mouse Monoclonal Antibody [13G2]
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
CD42b Mouse Monoclonal Antibody [13G2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human CD42b aa 100-350 / 652.
Species Reactivity
Human
Validated Applications
IHC-P, FC
Molecular Weight
Predicted band size: 72 kDa
Positive Control
Human spleen tissue, human bone marrow tissue, Jurkat.
Conjugation
unconjugated
Clone Number
13G2
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:1,000
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FC
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1:50-1:100
Target
Function
Glycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that is linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF). The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Mutations in this gene result in Bernard-Soulier syndromes and platelet-type von Willebrand disease. The coding region of this gene is known to contain a polymophic variable number tandem repeat (VNTR) domain that is associated with susceptibility to nonarteritic anterior ischemic optic neuropathy.
Background References
1. Ku NK. et. al. Use of CD42b immunohistochemical stain for the detection of Histoplasma. Ann Diagn Pathol. 2018 Feb;32:47-50.
2. Mäyränpää MI. et. al. Improved identification of endothelial erosion by simultaneous detection of endothelial cells (CD31/CD34) and platelets (CD42b). Endothelium. 2007 Mar-Apr;14(2):81-7.
Post-translational Modification
Glycocalicin is the product of a proteolytic cleavage/shedding, catalyzed by ADAM17, which releases most of the extracellular domain. Binding sites for vWF and thrombin are in this part of the protein.
Subcellular Location
Membrane.
UNIPROT
Synonyms
Antigen CD42b alpha antibody
BDPLT1 antibody
BDPLT3 antibody
BSS antibody
CD 42b antibody
CD42b alpha antibody
CD42B antibody
CD42b antigen antibody
DBPLT3 antibody
GLYCOCALICIN antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD42b antibody (EM1902-15) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Mouse anti-CD42b antibody (EM1902-15) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of CD42b was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-15, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CD42b Recombinant Mouse Monoclonal Antibody [13G2-R]
Application: IHC-P
Reactivity: Human
Conjugate: unconjugated
Products with the same target and pathway
CD42b Recombinant Mouse Monoclonal Antibody [13G2-R]
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Reactivity: Human
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CD42b Recombinant Mouse Monoclonal Antibody [13G2-R] - BSA and Azide free
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