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CD42b Recombinant Mouse Monoclonal Antibody [13G2-R] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610202

CD42b Recombinant Mouse Monoclonal Antibody [13G2-R] - BSA and Azide free

Application

  • IHC-P

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CD42b Recombinant Mouse Monoclonal Antibody [13G2-R] - BSA and Azide free

Antibody Type

Recombinant Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human CD42b aa 100-350 / 652.

Species Reactivity

Human

Validated Applications

IHC-P

Molecular Weight

Predicted band size: 72 kDa

Positive Control

Human bone marrow tissue, human spleen tissue.

Conjugation

unconjugated

Clone Number

13G2-R

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:1,000

Target

Function

Glycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that is linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF). The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Mutations in this gene result in Bernard-Soulier syndromes and platelet-type von Willebrand disease. The coding region of this gene is known to contain a polymophic variable number tandem repeat (VNTR) domain that is associated with susceptibility to nonarteritic anterior ischemic optic neuropathy.

Background References

1. Ku NK. et. al. Use of CD42b immunohistochemical stain for the detection of Histoplasma. Ann Diagn Pathol. 2018 Feb;32:47-50.

2. Mäyränpää MI. et. al. Improved identification of endothelial erosion by simultaneous detection of endothelial cells (CD31/CD34) and platelets (CD42b). Endothelium. 2007 Mar-Apr;14(2):81-7.

Subcellular Location

Membrane.

UNIPROT

SwissProt: P07359 Human

Synonyms

Antigen CD42b alpha antibody

BDPLT1 antibody

BDPLT3 antibody

BSS antibody

CD 42b antibody

CD42b alpha antibody

CD42B antibody

CD42b antigen antibody

DBPLT3 antibody

GLYCOCALICIN antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Mouse anti-CD42b antibody (<a href="/products/HA610202" style="font-weight: bold;text-decoration: underline;">HA610202</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610202" style="font-weight: bold;text-decoration: underline;">HA610202</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Mouse anti-CD42b antibody (HA610202) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610202) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD42b antibody (<a href="/products/HA610202" style="font-weight: bold;text-decoration: underline;">HA610202</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610202" style="font-weight: bold;text-decoration: underline;">HA610202</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD42b antibody (HA610202) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610202) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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