CD44 Recombinant Rabbit Monoclonal Antibody [PSH28-99]
Catalog# HA724828
CD44 Recombinant Rabbit Monoclonal Antibody [PSH28-99]
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WB
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IF-Cell
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FC
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Human
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unconjugated
Overview
Product Name
CD44 Recombinant Rabbit Monoclonal Antibody [PSH28-99]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CD44 aa 1-250.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 82 kDa
Positive Control
HeLa (Human cervical adenocarcinoma cell) cell lysate, PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, MDA-MB-231 (Human breast cancer cell) cell lysate, HUVEC (Human umbilical vein endothelial cell) cell lysate, T98G (Human brain glioblastoma cell) cell lysate.
Conjugation
unconjugated
Clone Number
PSH28-99
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
0.5 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:1,000
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FC
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1:500
Target
Function
The CD44 antigen is a cell-surface glycoprotein involved in cell–cell interactions, cell adhesion and migration. In humans, the CD44 antigen is encoded by the CD44 gene on chromosome 11. CD44 has been referred to as HCAM (homing cell adhesion molecule), Pgp-1 (phagocytic glycoprotein-1), Hermes antigen, lymphocyte homing receptor, ECM-III, and HUTCH-1.
Background References
1. Weng X et al. The membrane receptor CD44: novel insights into metabolism. Trends Endocrinol Metab. 2022 May
2. Hassn Mesrati M et al. CD44: A Multifunctional Mediator of Cancer Progression. Biomolecules. 2021 Dec
Subcellular Location
Cell membrane, Cell projection, microvillus, Secreted.
UNIPROT
Synonyms
LHR antibody
BA-1 antibody
CD 44 antibody
CD44 antibody
CD44 antigen antibody
CD44 molecule (Indian blood group) antibody
CD44 molecule antibody
CD44_HUMAN antibody
CDw44 antibody
CDW44 antigen antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CD44 on different lysates with Rabbit anti-CD44 antibody (HA724828) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate
Lane 3: LNCaP (Human prostate cancer cell) cell lysate
Lane 4: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 5: MDA-MB-231 (Human breast cancer cell) cell lysate
Lane 6: HUVEC (Human umbilical vein endothelial cell) cell lysate
Lane 7: T98G (Human brain glioblastoma cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 7 seconds; ECL: K1801
The staining pattern is consistent with reference (PMID: 19507218, PMID: 20670691).
Negative control: LNCaP (PMID: 31331331)
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724828, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 82 kDa
Observed band size: 82 kDa -
☑ Relative expression (RE)
Application: Immunocytochemistry (IF-cell)
Species: Human
Sample: PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) / LNCaP (Human prostate cancer cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.
Primary antibody: HA724828, 1/1,000, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
Negative control: LNCaP (PMID: 31331331). -
☑ Relative expression (RE)
Application: Flow Cytometry
Species: Human
Sample: LNCaP (Human prostate cancer cell) (Left, Negative) / PANC-1 (Human epidermoid carcinoma pancreatic ductal cell)
Blocking: 1% BSA + 10% normal goat serum + human FcR (Miltenyi Biotec), 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724828 (1/500, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Negative control: LNCaP (PMID: 31331331).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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