CD55 Mouse Monoclonal Antibody [12F1]
Catalog# EM1902-42
CD55 Mouse Monoclonal Antibody [12F1]
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WB
-
FC
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Human
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unconjugated
Overview
Product Name
CD55 Mouse Monoclonal Antibody [12F1]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human CD55 aa 150- 381.
Species Reactivity
Human
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 41 kDa
Positive Control
A549 cell lysate, HeLa cell lysate, MDA-MB-231 cell lysate, SW1990.
Conjugation
unconjugated
Clone Number
12F1
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
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FC
-
1:50-1:100
Target
Function
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade . Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage .
Background References
1. Shafren D. R. et. al. Coxsackievirus A21 binds to decay-accelerating factor but requires intercellular adhesion molecule 1 for cell entry. J. Virol. 71:4736-4743(1997).
Subcellular Location
Cell membrane, Membrane, Secreted.
UNIPROT
Synonyms
CD 55 antibody
CD55 antibody
CD55 antigen antibody
CD55 Cromer blood group system antibody
CD55 molecule (Cromer blood group) antibody
CD55 molecule antibody
CD55 molecule, decay accelerating factor for complement (Cromer blood group) antibody
Cd55a antibody
Complement decay accelerating factor antibody
Complement decay-accelerating factor antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CD55 on different lysates with Mouse anti-CD55 antibody (EM1902-42) at 1/500 dilution.
Lane 1: A549 cell lysate
Lane 2: LNCaP cell lysate (negative)
Lane 3: HeLa cell lysate
Lane 4: MDA-MB-231 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 70-75 kDa
Exposure time: 1 minute 2 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-42) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of CD55 was done on SW1990 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-42, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CD55 Recombinant Rabbit Monoclonal Antibody [JE31-59]
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
Products with the same target and pathway
CD55 Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P
Reactivity: Human
Conjugate: unconjugated