CD55 Rabbit Polyclonal Antibody
Catalog# ER1802-46
CD55 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
CD55 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human CD55 aa 130-381.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 42/49/60 kDa
Positive Control
Hela and PC-3M cell lysates, human plasma, human colon cancer tissue, human placenta tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
CD55, also called decay accelerating factor (DAF), is a GPI-anchored single chain glycoprotein. CD55 may play a role in protecting cells from complement-mediated lysis by preventing the amplification steps of the complement cascade. CD55 functions to prevent the assembly of C3 convertase or to accelerate the disassembly of preformed convertase, which blocks formation of the membrane attack complex. CD55 is expressed on cells in contact with serum, including hematopoietic and many non-hematopoietic cells.
Background References
暂无
Sequence Similarity
Belongs to the receptors of complement activation (RCA) family.
Tissue Specificity
Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Post-translational Modification
The Ser/Thr-rich domain is heavily O-glycosylated.
Subcellular Location
Cell membrane. Secreted.
UNIPROT
Synonyms
CD 55 antibody
CD55 antibody
CD55 antigen antibody
CD55 Cromer blood group system antibody
CD55 molecule (Cromer blood group) antibody
CD55 molecule antibody
CD55 molecule, decay accelerating factor for complement (Cromer blood group) antibody
Cd55a antibody
Complement decay accelerating factor antibody
Complement decay-accelerating factor antibody
ExpandImages
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Western blot analysis of CD55 on human plasma using anti-CD55 antibody at 1/1,000
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Western blot analysis of CD55 on Hela and PC-3M cell lysates using anti-CD55 antibody at 1/500 dilution.
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ICC staining CD55 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD55 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD55 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
CD55 Recombinant Rabbit Monoclonal Antibody [JE31-59]
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
Products with the same target and pathway
CD55 Mouse Monoclonal Antibody [12F1]
Application: WB,FC
Reactivity: Human
Conjugate: unconjugated