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CD90 / THY1 Recombinant Rabbit Monoclonal Antibody [SU35-07]

Usd: 205

Specification

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Catalog# ET1608-46

CD90 / THY1 Recombinant Rabbit Monoclonal Antibody [SU35-07]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD90 / THY1 Recombinant Rabbit Monoclonal Antibody [SU35-07]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CD90 aa 50-99 / 161.

Species Reactivity

Human, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 18 kDa

Positive Control

Human brain tissue lysate, Rat brain tissue lysate, human brain tissue, human breast cancer tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

SU35-07

RRID

AB_3069807

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Thy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.

Background References

1. Do an, A. et al. 2015. In vitro differentiation of human tooth germ stem cells into endothelial- and epithelial-like cells. Cell biology international. 39: 94-103.

2. Demirci, S. et al. 2014. Boron increases the cell viability of mesenchymal stem cells after long-term cryopreservation. Cryobiology. 68: 139-46.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P04216 Human

SwissProt: P01830 Rat

Synonyms

CD7 antibody

CD90 antibody

CD90 antigen antibody

CDw90 antibody

FLJ33325 antibody

MGC128895 antibody

T25 antibody

Theta antigen antibody

Thy 1 antibody

Thy 1 cell surface antigen antibody

Expand

Images

  • Western blot analysis of CD90 / THY1 on different lysates with Rabbit anti-CD90 / THY1 antibody (<a href="/products/ET1608-46" style="font-weight: bold;text-decoration: underline;">ET1608-46</a>) at 1/2,000 dilution.<br /><br />Lane 1: Human brain tissue lysate (20 µg/Lane)<br />Lane 2: Rat brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 18 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 2 minutes 20 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-46" style="font-weight: bold;text-decoration: underline;">ET1608-46</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD90 / THY1 on different lysates with Rabbit anti-CD90 / THY1 antibody (ET1608-46) at 1/2,000 dilution.

    Lane 1: Human brain tissue lysate (20 µg/Lane)
    Lane 2: Rat brain tissue lysate (20 µg/Lane)

    Predicted band size: 18 kDa
    Observed band size: 25 kDa

    Exposure time: 2 minutes 20 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-46) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-CD90 / THY1 antibody (<a href="/products/ET1608-46" style="font-weight: bold;text-decoration: underline;">ET1608-46</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1608-46" style="font-weight: bold;text-decoration: underline;">ET1608-46</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-CD90 / THY1 antibody (ET1608-46) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-46) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CD90 / THY1 antibody (<a href="/products/ET1608-46" style="font-weight: bold;text-decoration: underline;">ET1608-46</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1608-46" style="font-weight: bold;text-decoration: underline;">ET1608-46</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CD90 / THY1 antibody (ET1608-46) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-46) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-CD90 / THY1 antibody (<a href="/products/ET1608-46" style="font-weight: bold;text-decoration: underline;">ET1608-46</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1608-46" style="font-weight: bold;text-decoration: underline;">ET1608-46</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-CD90 / THY1 antibody (ET1608-46) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-46) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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