CD90 / THY1 Recombinant Rabbit Monoclonal Antibody [JF10-09]
Overview
Product Name
CD90 / THY1 Recombinant Rabbit Monoclonal Antibody [JF10-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human THY1 aa 75-120 / 161.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P, IHC-Fr
Molecular Weight
Predicted band size: 18 kDa
Positive Control
Hela cell lysate, HepG2 cell lysate, HUVEC cell lysate, human lung carcinoma tissue, human kidney tissue, human tonsil tissue, human spleen tissue, mouse brain tissue, mouse hippocampus tissue.
Conjugation
unconjugated
Clone Number
JF10-09
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
-
IF-Cell
-
1:50-1:200
-
IF-Tissue
-
1:400-1:1,000
-
IHC-P
-
1:2,000-1:4,000
-
IHC-Fr
-
1:100-1:200
Target
Function
Over 100 cell surface markers have been identified through the use of monoclonal antibodies. Many of these markers have proven useful in identifying specific subpopulations of cells within mixed colonies. Accordingly, these molecules have been assigned a "cluster of differentiation" (CD) designation. One such marker, designated Thy-1 (also referred to as CDw90), is a phosphatidyl-anchored cell surface glycoprotein which, when coexpressed with CD34 on cells from normal human bone marrow, identifies a subpopulation that includes putative hematopoietic, pleuripotent stem cells. Thy-1+ cells from bone marrow have been implicated in syngeneic graft versus host disease and may serve to regulate autoreactivity after bone marrow transplant.
Background References
1. Chen WC et al. Cancer stem cell marker CD90 inhibits ovarian cancer formation via 3 integrin. Int J Oncol 49:1881-1889 (2016).
2. Du M et al. Acellular dermal matrix loading with bFGF achieves similar acceleration of bone regeneration to BMP-2 via differential effects on recruitment, proliferation and sustained osteodifferentiation of mesenchymal stem cells. Mater Sci Eng C Mater Biol Appl 70:62-70 (2017).
Subcellular Location
Cell membrane.
Synonyms
CD7 antibody
CD90 antibody
CD90 antigen antibody
CDw90 antibody
FLJ33325 antibody
MGC128895 antibody
T25 antibody
Theta antigen antibody
Thy 1 antibody
Thy 1 cell surface antigen antibody
ExpandImages
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Western blot analysis of CD90 / THY1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HUVEC cell lysate -
![<span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />All lanes: Western blot analysis of THY1 with anti-CD90 / THY1 antibody[JF10-09] (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>) at 1/500 dilution.<br />Lane 1: Wild-type Hela whole cell lysate (10 µg).<br />Lane 2: THY1 knockdown Hela whole cell lysate (10 µg).<br /><br /><a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a> was shown to specifically react with THY1 in wild-type Hela cells. Weakened bands were observed when THY1 knockdown samples were tested. Wild-type and THY1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-92" style="font-weight: bold;text-decoration: underline;">ET1702-92</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.](https://storage.huabio.cn/huabio/productImg/ET1702-92_2.jpg?v=20251208104622)
☑ Knockdown (KD)
All lanes: Western blot analysis of THY1 with anti-CD90 / THY1 antibody[JF10-09] (ET1702-92) at 1/500 dilution.
Lane 1: Wild-type Hela whole cell lysate (10 µg).
Lane 2: THY1 knockdown Hela whole cell lysate (10 µg).
ET1702-92 was shown to specifically react with THY1 in wild-type Hela cells. Weakened bands were observed when THY1 knockdown samples were tested. Wild-type and THY1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling CD90 / THY1 with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-92) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-92) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-92) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Rabbit anti-CD90 / THY1 antibody (ET1702-92) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-92) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/100
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1/100
Antigen retrieval: Not required -
Application: IF-tissue
Species: Human
Site: Kidney
Sample: Paraffin-embedded section
Antibody concentration: 1/400 -
Application: IF-tissue
Species: Mouse
Site: Cerebral cortex
Sample: Paraffin-embedded section
Antibody concentration: 1/400
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
LAMA4+ CD90+ eCAFs provide immunosuppressive microenvironment for liver cancer through induction of CD8+ T cell senescence
Journal: Cell Communication And Signaling
DOI: 10.1186/s12964-025-02162-7
IF: 8.2
Application: IHC-P
Reactivity: Human
Publish date: 2025 Apr
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