CEACAM6 Rabbit Polyclonal Antibody
Catalog# ER1902-54
CEACAM6 Rabbit Polyclonal Antibody
-
WB
-
Human
-
unconjugated
Overview
Product Name
CEACAM6 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human CEACAM6 aa 49-224 / 344.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 37 kDa
Positive Control
BxPC-3 cell lysate, LoVo cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Cell surface glycoprotein that plays a role in cell adhesion and tumor progression . Intercellular adhesion occurs in a calcium- and fibronectin-independent manner. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8 . Heterophilic interaction with CEACAM8 occurs in activated neutrophils . Plays a role in neutrophil adhesion to cytokine-activated endothelial cells . Plays a role as an oncogene by promoting tumor progression; positively regulates cell migration, cell adhesion to endothelial cells and cell invasion . Also involved in the metastatic cascade process by inducing gain resistance to anoikis of pancreatic adenocarcinoma and colorectal carcinoma cells .
Background References
1. Oikawa S et al. Cell adhesion activity of non-specific cross-reacting antigen (NCA) and carcinoembryonic antigen (CEA) expressed on CHO cell surface: homophilic and heterophilic adhesion. Biochem Biophys Res Commun 164:39-45 (1989) .
Sequence Similarity
Belongs to the immunoglobulin superfamily. CEA family.
Tissue Specificity
Expressed in neutrophils. Expressed in columnar epithelial and goblet cells of the colon. Expressed in numerous tumor cell lines (at protein level).
Post-translational Modification
Glycosylated.
Subcellular Location
Cell membrane, Membrane.
UNIPROT
Synonyms
Carcinoembryonic antigen related cell adhesion molecule 6 antibody
Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) antibody
Carcinoembryonic antigen-related cell adhesion molecule 6 antibody
CD 66c antibody
CD66c antibody
CD66c antigen antibody
CEA LIKE PROTEIN antibody
CEACAM 6 antibody
CEACAM6 antibody
CEAL antibody
ExpandImages
-
☑ Relative expression (RE)
Western blot analysis of CEACAM6 on different lysates with Rabbit anti-CEACAM6 antibody (ER1902-54) at 1/1,000 dilution.
Lane 1: BxPC-3 cell lysate
Lane 2: LNCaP cell lysate (negative)
Lane 3: LoVo cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 50-200 kDa
Exposure time: 7 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-54) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CEACAM6 Mouse Monoclonal Antibody [A1E2]
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
