Biotin Conjugated Human CEACAM6 Recombinant Rabbit Monoclonal Antibody [PSH27-95] - Detector

Sandwich ELISA analysis of Human CEACAM6 protein matched pair antibodies

Capture: HA724736, Human CEACAM6 Rabbit mAb [PSH27-94]
Detector: HA724737, Human CEACAM6 Rabbit mAb [PSH27-95]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724736) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CEACAM6 protein (HA211497) starting from 6,000 pg/mL to 0 pg/mL and detect antibody (HA724737, Biotin, 0.05 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CEACAM6 in human samples.

Capture: HA724736, Human CEACAM6 Rabbit mAb [PSH27-94]
Detector: HA724737, Human CEACAM6 Rabbit mAb [PSH27-95]

Interpolated concentration of native CEACAM6 was measured in duplicate at different sample concentrations and interpolated from the CEACAM6 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CEACAM6 concentration was determined to be 46,566 pg/mL in human serum. There was no detectable signal in A-172 cell supnatant.

Interpolated concentrations of native CEACAM6 in BxPC-3 Cell extract samples based on a 1,000 µg/mL extract load.

Capture: HA724736, Human CEACAM6 Rabbit mAb [PSH27-94]
Detector: HA724737, Human CEACAM6 Rabbit mAb [PSH27-95]

Interpolated concentration of native CEACAM6 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CEACAM6 concentration was determined to be 149,767 pg/mL in BxPC-3 Cell extract.

Interpolated concentrations of spiked CEACAM6 in cell culture media samples.

Capture: HA724736, Human CEACAM6 Rabbit mAb [PSH27-94]
Detector: HA724737, Human CEACAM6 Rabbit mAb [PSH27-95]

The concentrations of CEACAM6 were measured in duplicates, interpolated from the CEACAM6 standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).