COX2 Mouse Monoclonal Antibody
Catalog# RT1159
COX2 Mouse Monoclonal Antibody
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WB
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IP
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IF
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IHC-P
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ELISA
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
COX2 Mouse Monoclonal Antibody
Antibody Type
Mouse Monoclonal Antibody
Immunogen
peptide
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP, IF, IHC-P, ELISA
Molecular Weight
70-72kDa
Positive Control
RAW 264.7, NIH/3T3, human gall bladder tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃
Storage Buffer
1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Isotype
IgG1
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:100-1:1000
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IP
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1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
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IF
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1:50-1:500
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IHC-P
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1:50-1:500
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ELISA
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1:30-1:3000
Target
Function
Prostaglandins are a diverse group of autocrine and paracrine hormones thatmediate many cellular and physiologic processes. Prostaglandin H2 (PGH2) isan intermediate molecule in formation of the prostaglandins. Cyclooxygenase-1(Cox-1) and cyclooxygenase-2 (Cox-2) are prostaglandin synthases that catalyzethe formation of PGH2 from arachidonic acid (AA). Cox-1 and Cox-2 areisozymes of prostaglandin-endoperoxidase synthase (PTGS). Cox-1 is constitutivelyexpressed in most tissues and is thought to serve in general “housekeeping”functions. Cox-2 is efficiently induced in migratory cells respondingto pro-inflammatory stimuli and is considered to be an important mediator ofinflammation. Both enzymes are targets for the nonsteroidal therapeutic antiinflammatorydrugs, NSAIDs.
Background References
1. Chen, Y.F., et al. 2013. High expression levels of COX-2 and P300 are associated with unfavorable survival in laryngeal squamous cell carcinoma. Eur. Arch. Otorhinolaryngol. 270: 1009-1017.
2. Ahmad, S., et al. 2012. Neuroprotective effect of sesame seed oil in 6-hydroxydopamine induced neurotoxicity in mice model: cellular, biochemical and neurochemical evidence. Neurochem. Res. 37: 516-526.
Subcellular Location
Microsome membrane, Endoplasmic reticulum membrane
UNIPROT
Synonyms
COX 2 antibody
COX-2 antibody
COX2 antibody
Cyclooxygenase 2 antibody
Cyclooxygenase 2b antibody
Cyclooxygenase antibody
Cyclooxygenase-2 antibody
Cyclooxygenase2 antibody
EC 1.14.99.1 antibody
fj02a10 antibody
ExpandImages
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Western blot analysis of Cox-2 expression in untreated (A), LPS treated (B) and LPS and Hydrocortisone treated (C) RAW 264.7 whole cell lysates. Note decreased LPS induction of Cox-2 expression in lane C.expression in lane C.
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Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing membrane and nuclear envelope localization (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells (B).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Ginsenoside Rg1 attenuates PM2.5-induced neurotoxicity by suppressing ferroptosis via the Nrf2/GPx4 axis
Journal: Journal Of Ethnopharmacology
DOI: 10.1016/j.jep.2026.121464
IF: 5.4
Application: WB
Reactivity: Mouse
Publish date: 2026 Mar
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Ginsenoside Rb1 Alleviates Atherosclerosis by Modulating Vascular Smooth Muscle Cell Proliferation, Foam Cell Formation, and Autophagy
Journal: Phytomedicine
DOI: 10.1016/j.phymed.2025.157503
IF: 8.3
Application: WB
Reactivity: Mouse
Publish date: 2025 Nov
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TLR4 interaction with PIEZO1 facilitates the 5-HT-mediated intestinal motility dysfunction in offspring mice induced by LPS exposure during pregnancy
Journal: Genes & Diseases
DOI: 10.1016/j.gendis.2025.101707
IF: 9.4
Application: WB
Reactivity: Mouse
Publish date: 2025 Jun
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Dehydroepiandrosterone ameliorates primary dysmenorrhea by suppressing the SP1/Hsp90ab1/COX-2 signaling pathway
Journal: Bioorganic Chemistry
DOI: 10.1016/j.bioorg.2025.108235
IF: 4.5
Application: IHC-P
Reactivity: Mouse
Publish date: 2025 Feb
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Colostrum-derived extracellular vesicles: potential multifunctional nanomedicine for alleviating mastitis
Journal: Journal Of Nanobiotechnology
DOI:
IF: 10.6
Application: WB
Reactivity: Mouse
Publish date: 2024 Oct
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Identification of potential anti-inflammatory components in Moutan Cortex by bio-affinity ultrafiltration coupled with ultra-performance liquid chromatography mass spectrometry
Journal: Frontiers In Pharmacology
DOI: 10.3389/fphar.2024.1358640
IF: 4.4
Application: WB
Reactivity: Mouse
Publish date: 2024 Feb
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Rapid identifying of COX-2 inhibitors from turmeric (Curcuma longa) by bioaffinity ultrafiltration coupled with UPLC-Q Exactive-Orbitrap-MS and zebrafish-based in vivo validation
Journal: Bioorganic Chemistry
DOI:
IF: 5.1
Application: WB
Reactivity: Mouse
Publish date: 2024 Apr
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Effect of taking aspirin before diagnosis on the prognosis of esophageal squamous cell carcinoma and analysis of prognostic factors
Journal: Journal Of International Medical Research
DOI:
IF: 1.671
Application: IHC-P
Reactivity: Human
Publish date: 2022 Apr
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Moderate mechanical stimulation rescues degenerative annulus fibrosus by suppressing caveolin-1 mediated pro-inflammatory signaling pathway. International journal of biological sciences, 17(5), 1395–1412.
Journal: International Journal Of Biological Sciences
DOI:
IF: 4.858
Application: IHC-P
Reactivity: Human
Publish date: 2021 Apr
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