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COX5A Recombinant Rabbit Monoclonal Antibody [PSH23-69]

Usd: 385

Specification

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Catalog# HA724392

COX5A Recombinant Rabbit Monoclonal Antibody [PSH23-69]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

COX5A Recombinant Rabbit Monoclonal Antibody [PSH23-69]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human COX5A aa 1-150.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 17 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cells) cell lysate, Hep G2 (Human liver cancer cells) cell lysate, A431 (Human epidermoid carcinoma skin squamous cells) cell lysate, HEK-293 (Human embryonic kidney cells) cell lysate, Mouse heart tissue lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Mouse kidney tissue lysate, Rat heart tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, Rat kidney tissue lysate.

Conjugation

unconjugated

Clone Number

PSH23-69

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Cytochrome c oxidase subunit 5a is a protein that in humans is encoded by the COX5A gene. Cytochrome c oxidase 5A is a subunit of the cytochrome c oxidase complex, also known as Complex IV, the last enzyme in the mitochondrial electron transport chain. Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane to drive ATP synthesis via protonmotive force. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex.

Background References

1. Zhang P et al. COX5A Alleviates Doxorubicin-Induced Cardiotoxicity by Suppressing Oxidative Stress, Mitochondrial Dysfunction and Cardiomyocyte Apoptosis. Int J Mol Sci. 2023 Jun

2. Cai M et al. COX5A as a potential biomarker for disease activity and organ damage in lupus. Clin Exp Med. 2023 Dec

Subcellular Location

Mitochondrion inner membrane.

UNIPROT

SwissProt: P20674 Human

SwissProt: P12787 Mouse

SwissProt: P11240 Rat

Synonyms

COX antibody

COX VA antibody

COX5A antibody

COX5A_HUMAN antibody

Cytochrome c oxidase polypeptide Va antibody

Cytochrome c oxidase polypeptide, mitochondrial antibody

Cytochrome c oxidase subunit 5A antibody

Cytochrome c oxidase subunit 5A, mitochondrial antibody

Cytochrome c oxidase subunit Va antibody

mitochondrial antibody

Expand

Images

  • Western blot analysis of COX5A on different lysates with Rabbit anti-COX5A antibody (<a href="/products/HA724392" style="font-weight: bold;text-decoration: underline;">HA724392</a>) at 1/5,000 dilution. <br /> <br />Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate <br />Lane 2: Hep G2 (Human liver cancer cells) cell lysate <br />Lane 3: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate <br />Lane 4: HEK-293 (Human embryonic kidney cells) cell lysate <br /> <br />Lysates/proteins at 20 µg/Lane. <br />Exposure time: 10 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724392" style="font-weight: bold;text-decoration: underline;">HA724392</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 17 kDa <br />Observed band size: 13 kDa

    Western blot analysis of COX5A on different lysates with Rabbit anti-COX5A antibody (HA724392) at 1/5,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
    Lane 2: Hep G2 (Human liver cancer cells) cell lysate
    Lane 3: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate
    Lane 4: HEK-293 (Human embryonic kidney cells) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724392, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 17 kDa
    Observed band size: 13 kDa

  • Western blot analysis of COX5A on different lysates with Rabbit anti-COX5A antibody (<a href="/products/HA724392" style="font-weight: bold;text-decoration: underline;">HA724392</a>) at 1/5,000 dilution. <br /> <br />Lane 1: Mouse heart tissue lysate <br />Lane 2: Mouse liver tissue lysate <br />Lane 3: Mouse brain tissue lysate <br />Lane 4: Mouse kidney tissue lysate <br />Lane 5: Rat heart tissue lysate <br />Lane 6: Rat liver tissue lysate <br />Lane 7: Rat brain tissue lysate <br />Lane 8: Rat kidney tissue lysate <br /> <br />Lysates/proteins at 40 µg/Lane. <br />Exposure time: 10 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA724392" style="font-weight: bold;text-decoration: underline;">HA724392</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 17 kDa <br />Observed band size: 13 kDa

    Western blot analysis of COX5A on different lysates with Rabbit anti-COX5A antibody (HA724392) at 1/5,000 dilution.

    Lane 1: Mouse heart tissue lysate
    Lane 2: Mouse liver tissue lysate
    Lane 3: Mouse brain tissue lysate
    Lane 4: Mouse kidney tissue lysate
    Lane 5: Rat heart tissue lysate
    Lane 6: Rat liver tissue lysate
    Lane 7: Rat brain tissue lysate
    Lane 8: Rat kidney tissue lysate

    Lysates/proteins at 40 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA724392, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 17 kDa
    Observed band size: 13 kDa

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Brain <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724392" style="font-weight: bold;text-decoration: underline;">HA724392</a>, 1/200, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724392, 1/200, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Heart <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA724392" style="font-weight: bold;text-decoration: underline;">HA724392</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1119" style="font-weight: bold;text-decoration: underline;">HA1119</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Heart
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA724392, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1119, 20 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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