COX5A Recombinant Rabbit Monoclonal Antibody [PSH23-69] - BSA and Azide free
Catalog# HA751908
COX5A Recombinant Rabbit Monoclonal Antibody [PSH23-69] - BSA and Azide free
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WB
-
IHC-P
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Human
-
Mouse
-
Rat
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HA724392
含抗保成分
-
unconjugated
Overview
Product Name
COX5A Recombinant Rabbit Monoclonal Antibody [PSH23-69] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human COX5A aa 1-150.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 17 kDa
Positive Control
HeLa (Human cervical adenocarcinoma cells) cell lysate, Hep G2 (Human liver cancer cells) cell lysate, A431 (Human epidermoid carcinoma skin squamous cells) cell lysate, HEK-293 (Human embryonic kidney cells) cell lysate, Mouse heart tissue lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Mouse kidney tissue lysate, Rat heart tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, Rat kidney tissue lysate.
Conjugation
unconjugated
Clone Number
PSH23-69
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IHC-P
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1:200-1:1,000
Target
Function
Cytochrome c oxidase subunit 5a is a protein that in humans is encoded by the COX5A gene. Cytochrome c oxidase 5A is a subunit of the cytochrome c oxidase complex, also known as Complex IV, the last enzyme in the mitochondrial electron transport chain. Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane to drive ATP synthesis via protonmotive force. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex.
Background References
1. Zhang P et al. COX5A Alleviates Doxorubicin-Induced Cardiotoxicity by Suppressing Oxidative Stress, Mitochondrial Dysfunction and Cardiomyocyte Apoptosis. Int J Mol Sci. 2023 Jun
2. Cai M et al. COX5A as a potential biomarker for disease activity and organ damage in lupus. Clin Exp Med. 2023 Dec
Subcellular Location
Mitochondrion inner membrane.
Synonyms
COX antibody
COX VA antibody
COX5A antibody
COX5A_HUMAN antibody
Cytochrome c oxidase polypeptide Va antibody
Cytochrome c oxidase polypeptide, mitochondrial antibody
Cytochrome c oxidase subunit 5A antibody
Cytochrome c oxidase subunit 5A, mitochondrial antibody
Cytochrome c oxidase subunit Va antibody
mitochondrial antibody
ExpandImages
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Western blot analysis of COX5A on different lysates with Rabbit anti-COX5A antibody (HA751908) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 2: Hep G2 (Human liver cancer cells) cell lysate
Lane 3: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate
Lane 4: HEK-293 (Human embryonic kidney cells) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751908, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 17 kDa
Observed band size: 13 kDa -
Western blot analysis of COX5A on different lysates with Rabbit anti-COX5A antibody (HA751908) at 1/5,000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse kidney tissue lysate
Lane 5: Rat heart tissue lysate
Lane 6: Rat liver tissue lysate
Lane 7: Rat brain tissue lysate
Lane 8: Rat kidney tissue lysate
Lysates/proteins at 40 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751908, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 17 kDa
Observed band size: 13 kDa -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751908, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Heart
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751908, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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COX5A Recombinant Rabbit Monoclonal Antibody [PSH23-69]
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