CRBN Recombinant Rabbit Monoclonal Antibody [PSH0-69]
Overview
Product Name
CRBN Recombinant Rabbit Monoclonal Antibody [PSH0-69]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CRBN aa 1-442 / 442.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP
Molecular Weight
Predicted band size: 51 kDa
Positive Control
HEK-293 cell lysate, A375 cell lysate, A549 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, NIH/3T3 cell lysate, MCF7 cell lysate, mouse brain tissue lysate, rat testis tissue lysate, human testis tissue, human brain tissue.
Conjugation
unconjugated
Clone Number
PSH0-69
RRID
Reactivity Data
| WB | IHC-P | IP | |
|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:200
-
IP
-
1-2μg/sample
Target
Function
Cereblon is a protein that in humans is encoded by the CRBN gene. The gene that encodes the cereblon protein is found on the human chromosome 3, on the short arm at position p26.3 from base pair 3,190,676 to base pair 3,221,394. CRBN orthologs are highly conserved from plants to humans. Cereblon forms an E3 ubiquitin ligase complex with damaged DNA binding protein 1 (DDB1), Cullin-4A (CUL4A), and regulator of cullins 1 (ROC1). This complex ubiquitinates a number of other proteins. Through a mechanism which has not been completely elucidated, this ubiquitination results in reduced levels of fibroblast growth factor 8 (FGF8) and fibroblast growth factor 10 (FGF10). FGF8 in turn regulates a number of developmental processes, such as limb and auditory vesicle formation. The net result is that this ubiquitin ligase complex is important for limb outgrowth in embryos. In the absence of cereblon, DDB1 forms a complex with DDB2 that functions as a DNA damage-binding protein. Furthermore, cereblon and DDB2 bind to DDB1 in a competitive manner.
Background References
1. Wang C et al. Developments of CRBN-based PROTACs as potential therapeutic agents. Eur J Med Chem. 2021 Dec
2. Yamamoto J et al. Discovery of CRBN as a target of thalidomide: a breakthrough for progress in the development of protein degraders. Chem Soc Rev. 2022 Aug
Subcellular Location
Cytoplasm, Nucleus, Membrane.
Synonyms
2610203G15Rik antibody
2900045O07Rik antibody
AF229032 antibody
AW108261 antibody
Cereblon antibody
Crbn antibody
CRBN_HUMAN antibody
DKFZp781K0715 antibody
MGC27358 antibody
MRT2A antibody
ExpandImages
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Western blot analysis of CRBN on different lysates with Rabbit anti-CRBN antibody (HA721457) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: A375 cell lysate
Lane 3: A549 cell lysate
Lane 4: HeLa cell lysate
Lane 5: SH-SY5Y cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: MCF7 cell lysate
Lane 8: Mouse brain tissue lysate
Lane 9: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721457) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CRBN antibody (HA721457) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721457) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-CRBN antibody (HA721457) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721457) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Knockdown (KD)
Western blot analysis of CRBN on different lysates with Rabbit anti-CRBN antibody (HA721457) at 1/1,000 dilution.
Lane 1: HeLa-si NT cell lysate
Lane 2: HeLa-si CRBN cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721457) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
CRBN was immunoprecipitated in 0.2mg HeLa cell lysate with HA721457 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721457 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA721457 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA721457 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 10 seconds; ECL: K1802
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Selenoprotein thioredoxin reductase 1 promotes cancer cells ferroptosis by suppressing GPX4 expression
Journal: Cell Death & Differentiation
DOI: 10.1038/s41418-026-01691-z
IF: 15.4
Application: WB
Reactivity: Human
Publish date: 2026 Feb