CREB1 Recombinant Rabbit Monoclonal Antibody [SA04-04] - BSA and Azide free (Capture)
Overview
Product Name
CREB1 Recombinant Rabbit Monoclonal Antibody [SA04-04] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CREB1 aa 246-264.
Species Reactivity
Human, Mouse, Rat
Validated Applications
ELISA(Cap)
Positive Control
Recombinant Mouse CREB1 protein (HA211601).
Conjugation
unconjugated
Clone Number
SA04-04
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH26-25] to CREB1 (Detecor) (HA724611) and Recombinant CREB1 protein (HA211601) as the standard. The reference range value is 78.1-20,000 pg/mL.
Target
Function
CAMP responsive element binding protein 1, also known as CREB-1, is a protein that in humans is encoded by the CREB1 gene. This protein binds the cAMP response element, a DNA nucleotide sequence present in many viral and cellular promoters. The binding of CREB1 stimulates transcription. This protein is a CREB transcription factor that is a member of the leucine zipper family of DNA-binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Background References
1. Zhao Y et al. Histone phosphorylation integrates the hepatic glucagon-PKA-CREB gluconeogenesis program in response to fasting. Mol Cell. 2023 Apr
2. Tang Y et al. Jiao-tai-wan and its effective component-berberine improve diabetes and depressive disorder through the cAMP/PKA/CREB signaling pathway. J Ethnopharmacol. 2024 Apr
Subcellular Location
Nucleus.
Synonyms
Active transcription factor CREB antibody
cAMP response element binding protein 1 antibody
cAMP response element binding protein antibody
cAMP responsive element binding protein 1 antibody
cAMP-responsive element-binding protein 1 antibody
CREB antibody
CREB-1 antibody
CREB1 antibody
CREB1_HUMAN antibody
Cyclic AMP-responsive element-binding protein 1 antibody
ExpandImages
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![Sandwich ELISA analysis of CREB1 matched pair antibodies<br /><br />Capture: <a href="/products/HA724610" style="font-weight: bold;text-decoration: underline;">HA724610</a>, CREB1 Rabbit mAb [SA04-04]<br />Detector: <a href="/products/HA724611" style="font-weight: bold;text-decoration: underline;">HA724611</a>, CREB1 Rabbit mAb [PSH26-25]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA724610" style="font-weight: bold;text-decoration: underline;">HA724610</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CREB1 protein (<a href="/products/HA211601" style="font-weight: bold;text-decoration: underline;">HA211601</a>) starting from 20,000 pg/mL to 0 pg/mL and detect antibody (<a href="/products/HA724611" style="font-weight: bold;text-decoration: underline;">HA724611</a>, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA724610_1.png?v=20260717091436)
Sandwich ELISA analysis of CREB1 matched pair antibodies
Capture: HA724610, CREB1 Rabbit mAb [SA04-04]
Detector: HA724611, CREB1 Rabbit mAb [PSH26-25]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724610) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse CREB1 protein (HA211601) starting from 20,000 pg/mL to 0 pg/mL and detect antibody (HA724611, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native CREB1 in HepG2, NIH/3T3 and C6 Cell extract samples based on a 1,000 µg/mL extract load.<br /><br />Capture: <a href="/products/HA724610" style="font-weight: bold;text-decoration: underline;">HA724610</a>, CREB1 Rabbit mAb [SA04-04]<br />Detector: <a href="/products/HA724611" style="font-weight: bold;text-decoration: underline;">HA724611</a>, CREB1 Rabbit mAb [PSH26-25]<br /><br />Interpolated concentration of native CREB1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CREB1 concentration was determined to be 29,085 pg/mL in HepG2 Cell extract,23,515 pg/mL in NIH/3T3 Cell extract and 24,845 pg/mL in C6 Cell extract.](https://storage.huabio.cn/huabio/productImg/HA724610_2.png?v=20260717091436)
Interpolated concentrations of native CREB1 in HepG2, NIH/3T3 and C6 Cell extract samples based on a 1,000 µg/mL extract load.
Capture: HA724610, CREB1 Rabbit mAb [SA04-04]
Detector: HA724611, CREB1 Rabbit mAb [PSH26-25]
Interpolated concentration of native CREB1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean CREB1 concentration was determined to be 29,085 pg/mL in HepG2 Cell extract,23,515 pg/mL in NIH/3T3 Cell extract and 24,845 pg/mL in C6 Cell extract. -
![Interpolated concentrations of spiked CREB1 in cell culture media samples.<br /><br />Capture: <a href="/products/HA724610" style="font-weight: bold;text-decoration: underline;">HA724610</a>, CREB1 Rabbit mAb [SA04-04]<br />Detector: <a href="/products/HA724611" style="font-weight: bold;text-decoration: underline;">HA724611</a>, CREB1 Rabbit mAb [PSH26-25]<br /><br />The concentrations of CREB1 were measured in duplicates, interpolated from the CREB1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA724610_3.png?v=20260717091436)
Interpolated concentrations of spiked CREB1 in cell culture media samples.
Capture: HA724610, CREB1 Rabbit mAb [SA04-04]
Detector: HA724611, CREB1 Rabbit mAb [PSH26-25]
The concentrations of CREB1 were measured in duplicates, interpolated from the CREB1 standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Biotin Conjugated CREB1 Recombinant Rabbit Monoclonal Antibody [PSH26-25] - Detector
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Reactivity: Human,Mouse,Rat
Conjugate: Biotin
CREB1 Recombinant Rabbit Monoclonal Antibody [PSH26-25] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
