CXCL1 / GRO alpha Recombinant Rabbit Monoclonal Antibody [PSH07-53]
Catalog# HA722841
CXCL1 / GRO alpha Recombinant Rabbit Monoclonal Antibody [PSH07-53]
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WB
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Human
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HA751174
不含抗保成分
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unconjugated
Overview
Product Name
CXCL1 / GRO alpha Recombinant Rabbit Monoclonal Antibody [PSH07-53]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CXCL1 aa 1-107.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 11 kDa
Positive Control
HUVEC treated with 0.5μg/mL LPS for 24 hours cell lysate, EA.hy926 treated with 0.5μg/mL LPS for 4 hours cell lysate.
Conjugation
unconjugated
Clone Number
PSH07-53
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4.
Background References
1. Collinson RJ, Mazza-Parton A, Fuller KA, Linden MD, Erber WN, Guo BB. Gene Expression of CXCL1 (GRO-α) and EGF by Platelets in Myeloproliferative Neoplasms. Hemasphere. 2020 Oct 26;4(6):e490. doi: 10.1097/HS9.0000000000000490. PMID: 33134870; PMCID: PMC7592996.
2. Zhou Y, Zhang J, Liu Q, Bell R, Muruve DA, Forsyth P, Arcellana-Panlilio M, Robbins S, Yong VW. The chemokine GRO-alpha (CXCL1) confers increased tumorigenicity to glioma cells. Carcinogenesis. 2005 Dec;26(12):2058-68. doi: 10.1093/carcin/bgi182. Epub 2005 Jul 20. PMID: 16033775.
Subcellular Location
Secreted.
UNIPROT
Synonyms
C-X-C motif chemokine 1 antibody
Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody
chemokine (C-X-C motif) ligand 1 antibody
CINC-1 antibody
CXCL1 antibody
Cytokine-induced neutrophil chemoattractant 1 antibody
Fibroblast secretory protein antibody
Fsp antibody
Gro 1 antibody
Gro A antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of CXCL1 / GRO alpha on different lysates with Rabbit anti-CXCL1 / GRO alpha antibody (HA722841) at 1/2,000 dilution.
Lane 1: HUVEC cell lysate
Lane 2: HUVEC treated with 0.5μg/mL LPS for 24 hours cell lysate, then add 300ng/mL BFA for 20 hours cell lysate
Lane 3: EA.hy926 cell lysate
Lane 4: EA.hy926 treated with 0.5μg/mL LPS for 4 hours cell lysate,
then add 300ng/mL BFA for 20 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722841) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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