CXCL1 / GRO alpha Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH07-53] (HA751174)
Catalog# HA751174
Rabbit Monoclonal to CXCL1 / GRO alpha
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of CXCL1 / GRO alpha on different lysates with Rabbit anti-CXCL1 / GRO alpha antibody (HA751174) at 1/2,000 dilution.
Lane 1: HUVEC cell lysate
Lane 2: HUVEC treated with 0.5μg/mL LPS for 24 hours cell lysate, then add 300ng/mL BFA for 20 hours cell lysate
Lane 3: EA.hy926 cell lysate
Lane 4: EA.hy926 treated with 0.5μg/mL LPS for 4 hours cell lysate,
then add 300ng/mL BFA for 20 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722841) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CXCL1 / GRO alpha on different lysates with Rabbit anti-CXCL1 / GRO alpha antibody (HA751174) at 1/2,000 dilution.
Lane 1: HUVEC cell lysate
Lane 2: HUVEC treated with 0.5μg/mL LPS for 24 hours cell lysate, then add 300ng/mL BFA for 20 hours cell lysate
Lane 3: EA.hy926 cell lysate
Lane 4: EA.hy926 treated with 0.5μg/mL LPS for 4 hours cell lysate,
then add 300ng/mL BFA for 20 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722841) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.