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Carcino Embryonic Antigen CEA Recombinant Rabbit Monoclonal Antibody [PSH18-46]

Usd: 385

Specification

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Catalog# HA723999

Carcino Embryonic Antigen CEA Recombinant Rabbit Monoclonal Antibody [PSH18-46]

Application

  • WB

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Carcino Embryonic Antigen CEA Recombinant Rabbit Monoclonal Antibody [PSH18-46]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 77 kDa

Positive Control

TT cell lysate, BxPC-3 cell lysate, human colon tissue.

Conjugation

unconjugated

Clone Number

PSH18-46

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000

Target

Function

Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) also known as CD66e (Cluster of Differentiation 66e), is a member of the carcinoembryonic antigen (CEA) gene family. In the literature, CEACAM5 is often used as a synonym for cancer embryonic antigen (CEA), a well-known biomarker of many types of malignancies, colorectal cancer in the first place. Its primary function in the embryonic intestine and colon tumors is adhesion between epithelial cells. Also, it plays a significant role in the inhibition of differentiation and apoptosis in colon cells. There are evidences that high CEACAM5 expression is firmly associated with the CD133-positive colorectal cancer stem cells.

Background References

1. Decary S et al. Preclinical Activity of SAR408701: A Novel Anti-CEACAM5-maytansinoid Antibody-drug Conjugate for the Treatment of CEACAM5-positive Epithelial Tumors. Clin Cancer Res. 2020 Dec

2. Wang X et al. CEACAM5 inhibits the lymphatic metastasis of head and neck squamous cell carcinoma by regulating epithelial-mesenchymal transition via inhibiting MDM2. Clin Sci (Lond). 2022 Nov

Subcellular Location

Cell membrane, Apical cell membrane, Cell surface.

UNIPROT

SwissProt: P06731 Human

Synonyms

Carcinoembryonic antigen antibody

Carcinoembryonic antigen-related cell adhesion molecule 5 antibody

CD66e antibody

CEA antibody

Ceacam5 antibody

CEAM5_HUMAN antibody

DKFZp781M2392 antibody

Meconium antigen 100 antibody

OTTHUMP00000199032 antibody

OTTHUMP00000199033 antibody

Expand

Images

  • Western blot analysis of Carcino Embryonic Antigen CEA on different lysates with Rabbit anti-Carcino Embryonic Antigen CEA antibody (<a href="/products/HA723999" style="font-weight: bold;text-decoration: underline;">HA723999</a>) at 1/5,000 dilution.<br /><br />Lane 1: TT cell lysate<br />Lane 2: BxPC-3 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 77 kDa<br />Observed band size: 180 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723999" style="font-weight: bold;text-decoration: underline;">HA723999</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Carcino Embryonic Antigen CEA on different lysates with Rabbit anti-Carcino Embryonic Antigen CEA antibody (HA723999) at 1/5,000 dilution.

    Lane 1: TT cell lysate
    Lane 2: BxPC-3 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 77 kDa
    Observed band size: 180 kDa

    Exposure time: 25 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723999) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Carcino Embryonic Antigen CEA antibody (<a href="/products/HA723999" style="font-weight: bold;text-decoration: underline;">HA723999</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723999" style="font-weight: bold;text-decoration: underline;">HA723999</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Carcino Embryonic Antigen CEA antibody (HA723999) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723999) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-Carcino Embryonic Antigen CEA antibody (<a href="/products/HA723999" style="font-weight: bold;text-decoration: underline;">HA723999</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723999" style="font-weight: bold;text-decoration: underline;">HA723999</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-Carcino Embryonic Antigen CEA antibody (HA723999) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723999) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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