Carcino Embryonic Antigen CEA Recombinant Rabbit Monoclonal Antibody [PSH18-46]
Catalog# HA723999
Carcino Embryonic Antigen CEA Recombinant Rabbit Monoclonal Antibody [PSH18-46]
-
WB
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
Carcino Embryonic Antigen CEA Recombinant Rabbit Monoclonal Antibody [PSH18-46]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 77 kDa
Positive Control
TT cell lysate, BxPC-3 cell lysate, human colon tissue.
Conjugation
unconjugated
Clone Number
PSH18-46
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IHC-P
-
1:1,000
Target
Function
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) also known as CD66e (Cluster of Differentiation 66e), is a member of the carcinoembryonic antigen (CEA) gene family. In the literature, CEACAM5 is often used as a synonym for cancer embryonic antigen (CEA), a well-known biomarker of many types of malignancies, colorectal cancer in the first place. Its primary function in the embryonic intestine and colon tumors is adhesion between epithelial cells. Also, it plays a significant role in the inhibition of differentiation and apoptosis in colon cells. There are evidences that high CEACAM5 expression is firmly associated with the CD133-positive colorectal cancer stem cells.
Background References
1. Decary S et al. Preclinical Activity of SAR408701: A Novel Anti-CEACAM5-maytansinoid Antibody-drug Conjugate for the Treatment of CEACAM5-positive Epithelial Tumors. Clin Cancer Res. 2020 Dec
2. Wang X et al. CEACAM5 inhibits the lymphatic metastasis of head and neck squamous cell carcinoma by regulating epithelial-mesenchymal transition via inhibiting MDM2. Clin Sci (Lond). 2022 Nov
Subcellular Location
Cell membrane, Apical cell membrane, Cell surface.
UNIPROT
Synonyms
Carcinoembryonic antigen antibody
Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
CD66e antibody
CEA antibody
Ceacam5 antibody
CEAM5_HUMAN antibody
DKFZp781M2392 antibody
Meconium antigen 100 antibody
OTTHUMP00000199032 antibody
OTTHUMP00000199033 antibody
ExpandImages
-
Western blot analysis of Carcino Embryonic Antigen CEA on different lysates with Rabbit anti-Carcino Embryonic Antigen CEA antibody (HA723999) at 1/5,000 dilution.
Lane 1: TT cell lysate
Lane 2: BxPC-3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 77 kDa
Observed band size: 180 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723999) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Carcino Embryonic Antigen CEA antibody (HA723999) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723999) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-Carcino Embryonic Antigen CEA antibody (HA723999) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723999) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Carcino Embryonic Antigen CEA Rabbit Polyclonal Antibody
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
Carcino Embryonic Antigen CEA Recombinant Rabbit Monoclonal Antibody [JM93-28]
Application: WB,IF-Cell,IF-Tissue,IHC-P
Reactivity: Human
Conjugate: unconjugated
Carcino Embryonic Antigen CEA Recombinant Rabbit Monoclonal Antibody [PSH18-46] - BSA and Azide free
Application: WB,IHC-P
Reactivity: Human
Conjugate: unconjugated
