Carcino Embryonic Antigen CEA Recombinant Rabbit Monoclonal Antibody [JM93-28] - BSA and Azide free

This data was developed using ET1705-52, the same antibody clone in a different buffer formulation.
Western blot analysis of Carcino Embryonic Antigen CEA on different lysates with Rabbit anti-Carcino Embryonic Antigen CEA antibody (ET1705-52) at 1/1,000 dilution.

Lane 1: A549 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: BxPC-3 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 77 kDa
Observed band size: 70-200 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-52) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

This data was developed using ET1705-52, the same antibody clone in a different buffer formulation.
ICC staining of Carcino Embryonic Antigen CEA in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-52, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

This data was developed using ET1705-52, the same antibody clone in a different buffer formulation.
ICC staining of Carcino Embryonic Antigen CEA in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-52, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

This data was developed using ET1705-52, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Carcino Embryonic Antigen CEA antibody (ET1705-52) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1705-52) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

This data was developed using ET1705-52, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Carcino Embryonic Antigen CEA antibody (ET1705-52) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1705-52) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.