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Cardiac Troponin T Mouse Monoclonal Antibody [3-G6]

Usd: 350

Specification

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Catalog# EM1701-39

Cardiac Troponin T Mouse Monoclonal Antibody [3-G6]

Application

  • WB

  • IHC-P

  • IF-Cell

  • IF-Tissue

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Cardiac Troponin T Mouse Monoclonal Antibody [3-G6]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within huamn Cardiac Troponin T aa 45-89/298.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Cell, IF-Tissue

Molecular Weight

Predicted band size: 36 kDa

Positive Control

Human heart tissue lysates, mouse heart tissue, human heart tissue, mouse heart tissue.

Conjugation

unconjugated

Clone Number

3-G6

RRID

AB_3068763

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2b

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • IF-Tissue

  • 1:500

Target

Function

Cardiac muscle troponin T (cTnT) is a protein that in humans is encoded by the TNNT2 gene. Cardiac TnT is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration.The TMNT2 gene is located at 1q32 in the human chromosomal genome, encoding the cardiac muscle isoform of troponin T (cTnT). Human cTnT is an ~36-kDa protein consisting of 297 amino acids including the first methionine with an isoelectric point (pI) of 4.88. It is the tropomyosin- binding and thin filament anchoring subunit of the troponin complex in cardiac muscle cells. TNNT2 gene is expressed in vertebrate cardiac muscles and embryonic skeletal muscles.

Background References

1. Petropoulou E et al. Digenic inheritance of mutations in the cardiac troponin (TNNT2) and cardiac beta myosin heavy chain (MYH7) as the cause of severe dilated cardiomyopathy. Eur J Med Genet. 60(9):485-488 (2017).

2. Li YD et al. TNNT2 Gene Polymorphisms are Associated with Susceptibility to Idiopathic Dilated Cardiomyopathy in Kazak and Han Chinese. Med Sci Monit. 3;21:3343-7 (2015).

Sequence Similarity

Belongs to the troponin T family.

Tissue Specificity

Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

Post-translational Modification

Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.

Subcellular Location

Cytoskeleton, Cytosol.

UNIPROT

SwissProt: P45379 Human

SwissProt: P50752 Mouse

Synonyms

Cardiac muscle troponin T antibody

Cardiomyopathy dilated 1D (autosomal dominant) antibody

Cardiomyopathy hypertrophic 2 antibody

CMD1D antibody

CMH2 antibody

CMPD2 antibody

cTnT antibody

LVNC6 antibody

MGC3889 antibody

OTTHUMP00000033864 antibody

Expand

Images

  • Western blot analysis of Cardiac Troponin T on human heart tissue lysate using anti- Cardiac Troponin T antibody at 1/1,000 dilution.

    Western blot analysis of Cardiac Troponin T on human heart tissue lysate using anti- Cardiac Troponin T antibody at 1/1,000 dilution.

  • ICC staining Cardiac Troponin T (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Cardiac Troponin T (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Cardiac Troponin T (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Cardiac Troponin T (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti- Cardiac Troponin T antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti- Cardiac Troponin T antibody. Counter stained with hematoxylin.

  • Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Cardiac Troponin T with Mouse anti-Cardiac Troponin T antibody (<a href="/products/EM1701-39" style="font-weight: bold;text-decoration: underline;">EM1701-39</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/EM1701-39" style="font-weight: bold;text-decoration: underline;">EM1701-39</a>, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Cardiac Troponin T with Mouse anti-Cardiac Troponin T antibody (EM1701-39) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM1701-39, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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