Cardiac Troponin T Recombinant Rabbit Monoclonal Antibody [SC64-03] - BSA and Azide free
Catalog# HA750222
Cardiac Troponin T Recombinant Rabbit Monoclonal Antibody [SC64-03] - BSA and Azide free
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
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ET1610-51
含抗保成分
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unconjugated
Overview
Product Name
Cardiac Troponin T Recombinant Rabbit Monoclonal Antibody [SC64-03] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Cardiac Troponin T aa 251-298/298.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 36 kDa
Positive Control
Mouse heart tissue lysate, Rat heart tissue lysate, human heart tissue, mouse heart tissue, rat heart tissue.
Conjugation
unconjugated
Clone Number
SC64-03
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:10,000
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IHC-P
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1:2,000
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IF-Tissue
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1:500
Target
Function
Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of two heavy chains (MHC) and four light chains (MLC) that interacts with Actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between Actin and Myosin by binding to calcium. Troponin is made up of at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C (the sensor), Troponin I (the regulator) and Troponin T (the link to the muscle thin filament). Troponin C is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has two isoforms: fast and slow. Fast Troponin C has two calcium binding sites, while slow/cardiac Troponin C has a single calcium binding site.
Background References
1. Szebényi K et al. Generation of a Homozygous Transgenic Rat Strain Stably Expressing a Calcium Sensor Protein for Direct Examination of Calcium Signaling. Sci Rep 5:12645 (2015).
2. Becker JR et al. Differential activation of natriuretic peptide receptors modulates cardiomyocyte proliferation during development. Development 141:335-45 (2014).
Sequence Similarity
Belongs to the troponin T family.
Tissue Specificity
Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.
Post-translational Modification
Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.
Subcellular Location
Cytoplasm, filament.
Synonyms
Cardiac muscle troponin T antibody
Cardiomyopathy dilated 1D (autosomal dominant) antibody
Cardiomyopathy hypertrophic 2 antibody
CMD1D antibody
CMH2 antibody
CMPD2 antibody
cTnT antibody
LVNC6 antibody
MGC3889 antibody
OTTHUMP00000033864 antibody
ExpandImages
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Western blot analysis of Cardiac Troponin T on different lysates with Rabbit anti-Cardiac Troponin T antibody (HA750222) at 1/2,000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: Rat heart tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 43 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750222) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Cardiac Troponin T antibody (HA750222) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750222) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Cardiac Troponin T antibody (HA750222) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750222) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Cardiac Troponin T antibody (HA750222) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750222) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Cardiac Troponin T with Rabbit anti-Cardiac Troponin T antibody (HA750222) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750222, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded mouse heart tissue labeling Cardiac Troponin T with Rabbit anti-Cardiac Troponin T antibody (HA750222) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750222, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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