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Caspase-12 Recombinant Rabbit Monoclonal Antibody [PSH07-49] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA751170

Caspase-12 Recombinant Rabbit Monoclonal Antibody [PSH07-49] - BSA and Azide free

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

Caspase-12 Recombinant Rabbit Monoclonal Antibody [PSH07-49] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse Caspase-12 aa 101-419.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 48 kDa

Positive Control

C2C12 cell lysate, SP2/0 cell lysate, human heart tissue, mouse heart tissue, rat heart tissue.

Conjugation

unconjugated

Clone Number

PSH07-49

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Caspase 12 is a protein that in humans is encoded by the CASP12 gene. The protein belongs to a family of enzymes called caspases which cleave their substrates at C-terminal aspartic acid residues. It is closely related to caspase 1 and other members of the caspase family, known as inflammatory caspases, which process and activate inflammatory cytokines such as interleukin 1 and interleukin 18.

Background References

1. Vesela B et al. Caspase-12 Is Present During Craniofacial Development and Participates in Regulation of Osteogenic Markers. Front Cell Dev Biol. 2020 Oct

2. Hetz C et al. Caspase-12 and endoplasmic reticulum stress mediate neurotoxicity of pathological prion protein. EMBO J. 2021 Sep

UNIPROT

SwissProt: Q6UXS9 Human

SwissProt: O08736 Mouse

SwissProt: Q920D5 Rat

Synonyms

CASP 12 antibody

CASP-12 antibody

Casp12 antibody

CASP12P1 antibody

caspase 12 (gene/pseudogene) antibody

caspase 12 pseudogene 1 antibody

CASPC_HUMAN antibody

Inactive caspase-12 antibody

OTTHUMP00000207032 antibody

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Caspase-12 on different lysates with Rabbit anti-Caspase-12 antibody (<a href="/products/HA751170" style="font-weight: bold;text-decoration: underline;">HA751170</a>) at 1/1,000 dilution.<br /><br />Lane 1: C2C12 cell lysate (20 µg/Lane)<br />Lane 2: J774A.1 cell lysate (negative) (20 µg/Lane)<br />Lane 3: SP2/0 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 48 kDa<br />Observed band size: 53 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA751170" style="font-weight: bold;text-decoration: underline;">HA751170</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Caspase-12 on different lysates with Rabbit anti-Caspase-12 antibody (HA751170) at 1/1,000 dilution.

    Lane 1: C2C12 cell lysate (20 µg/Lane)
    Lane 2: J774A.1 cell lysate (negative) (20 µg/Lane)
    Lane 3: SP2/0 cell lysate (20 µg/Lane)

    Predicted band size: 48 kDa
    Observed band size: 53 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751170) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Caspase-12 antibody (<a href="/products/HA751170" style="font-weight: bold;text-decoration: underline;">HA751170</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751170" style="font-weight: bold;text-decoration: underline;">HA751170</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Caspase-12 antibody (HA751170) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751170) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Caspase-12 antibody (<a href="/products/HA751170" style="font-weight: bold;text-decoration: underline;">HA751170</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751170" style="font-weight: bold;text-decoration: underline;">HA751170</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Caspase-12 antibody (HA751170) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751170) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Caspase-12 antibody (<a href="/products/HA751170" style="font-weight: bold;text-decoration: underline;">HA751170</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA751170" style="font-weight: bold;text-decoration: underline;">HA751170</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Caspase-12 antibody (HA751170) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751170) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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