Chromogranin A Recombinant Mouse Monoclonal Antibody [PD01-39]
Catalog# HA601103
Chromogranin A Recombinant Mouse Monoclonal Antibody [PD01-39]
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
Chromogranin A Recombinant Mouse Monoclonal Antibody [PD01-39]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant full length protein corresponding to Human Chromogranin A.
Species Reactivity
Human
Validated Applications
IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 51 kDa
Positive Control
Human pancreas tissue, human thyroid tissue, human atypical carcinoid tissue, human medullary thyroid carcinoma tissue, human appendix tissue.
Conjugation
unconjugated
Clone Number
PD01-39
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-P
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1:1,000-1:15,000
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IF-Tissue
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1:2,000
Target
Function
Chromogranin A or parathyroid secretory protein 1 (gene name CHGA) is a member of the granin family of neuroendocrine secretory proteins. As such, it is located in secretory vesicles of neurons and endocrine cells such as islet beta cell secretory granules in the pancreas. In humans, chromogranin A protein is encoded by the CHGA gene. Chromogranin A is the precursor to several functional peptides including vasostatin-1, vasostatin-2, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). Chromogranin A induces and promotes the generation of secretory granules such as those containing insulin in pancreatic islet beta cells.
Background References
1. Takada Y et al. Brg1 plays an essential role in development and homeostasis of the duodenum through regulation of Notch signaling. Development 143:3532-3539 (2016).
2. Simpson PD et al. Striking Oxygen Sensitivity of the Peptidylglycine a-Amidating Monooxygenase (PAM) in Neuroendocrine Cells. J Biol Chem 290:24891-901 (2015).
Sequence Similarity
Belongs to the chromogranin/secretogranin protein family.
Tissue Specificity
GE-25 is found in the brain.
Post-translational Modification
Sulfated on tyrosine residues and/or contains sulfated glycans.; O-glycosylated with core 1 or possibly core 8 glycans.; Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity. Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides.
Subcellular Location
Cytoplasmic vesicle, Secreted.
UNIPROT
Synonyms
beta Granin antibody
betagranin (N-terminal fragment of chromogranin A) antibody
catestatin antibody
CgA antibody
CHG A antibody
Chga antibody
chromofungin antibody
Chromogranin A parathyroid secretory protein 1 antibody
Chromogranin A precursor antibody
ChromograninA antibody
ExpandImages
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Application: IF-Tissue
Species: Human
Site: pancreas
Sample: Paraffin-embedded section
Antibody concentration: 1/2,000 -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Chromogranin A antibody (HA601103) at 1/15,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601103) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Mouse anti-Chromogranin A antibody (HA601103) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601103) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human atypical carcinoid tissue with Mouse anti-Chromogranin A antibody (HA601103) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601103) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human medullary thyroid carcinoma tissue with Mouse anti-Chromogranin A antibody (HA601103) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601103) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-Chromogranin A antibody (HA601103) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601103) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue (Negative control) with Mouse anti-Chromogranin A antibody (HA601103) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601103) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma tissue (Negative control) with Mouse anti-Chromogranin A antibody (HA601103) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601103) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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