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CrkL Recombinant Rabbit Monoclonal Antibody [JE50-21]

Usd: 385

Specification

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Catalog# HA721740

CrkL Recombinant Rabbit Monoclonal Antibody [JE50-21]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CrkL Recombinant Rabbit Monoclonal Antibody [JE50-21]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CrkL aa 254-303 / 303.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 34 kDa

Positive Control

K-562 cell lysate, A431 cell lysate, HT-29 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, rat cerebellum tissue, A431.

Conjugation

unconjugated

Clone Number

JE50-21

RRID

AB_3072852

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • FC

  • 1:1,000

Target

Function

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Background References

1. Guo C et al. CRKL promotes hepatocarcinoma through enhancing glucose metabolism of cancer cells via activating PI3K/Akt. J Cell Mol Med. 2021 Mar

2. Kim JH et al. Bifunctional Role of CrkL during Bone Remodeling. Int J Mol Sci. 2021 Jun

Subcellular Location

Cytosol, extrinsic component of postsynaptic membrane, neuromuscular junction, nucleoplasm.

UNIPROT

SwissProt: P46109 Human

SwissProt: P47941 Mouse

SwissProt: Q5U2U2 Rat

Synonyms

Crk L antibody

Crk like protein antibody

Crk-like protein antibody

Crkl antibody

CRKL_HUMAN antibody

Crkol antibody

HGNC:2363 antibody

Oncogene CrkL antibody

V crk avian sarcoma virus CT10 oncogene homolog like antibody

v crk sarcoma virus CT10 oncogene homolog (avian) like antibody

Expand

Images

  • Western blot analysis of CrkL on different lysates with Rabbit anti-CrkL antibody (<a href="/products/HA721740" style="font-weight: bold;text-decoration: underline;">HA721740</a>) at 1/1,000 dilution.<br /><br />Lane 1: K-562 cell lysate (20 µg/Lane)<br />Lane 2: A431 cell lysate (20 µg/Lane)<br />Lane 3: HT-29 cell lysate (20 µg/Lane)<br />Lane 4: Jurkat cell lysate (20 µg/Lane)<br />Lane 5: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 6: C6 cell lysate (20 µg/Lane)<br />Lane 7: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 8: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 34 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721740" style="font-weight: bold;text-decoration: underline;">HA721740</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CrkL on different lysates with Rabbit anti-CrkL antibody (HA721740) at 1/1,000 dilution.

    Lane 1: K-562 cell lysate (20 µg/Lane)
    Lane 2: A431 cell lysate (20 µg/Lane)
    Lane 3: HT-29 cell lysate (20 µg/Lane)
    Lane 4: Jurkat cell lysate (20 µg/Lane)
    Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 6: C6 cell lysate (20 µg/Lane)
    Lane 7: Mouse brain tissue lysate (40 µg/Lane)
    Lane 8: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 34 kDa
    Observed band size: 37 kDa

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721740) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-CrkL antibody (<a href="/products/HA721740" style="font-weight: bold;text-decoration: underline;">HA721740</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721740" style="font-weight: bold;text-decoration: underline;">HA721740</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-CrkL antibody (HA721740) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721740) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of A431 cells labeling CrkL.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721740" style="font-weight: bold;text-decoration: underline;">HA721740</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of A431 cells labeling CrkL.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721740, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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