Cullin-3 Rabbit Polyclonal Antibody
Catalog# ER1706-70
Cullin-3 Rabbit Polyclonal Antibody
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WB
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IHC-P
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
Cullin-3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human Cullin-3 aa 724-768.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 89 kDa
Positive Control
SH-SY5Y, 293T, mouse testis tissue lysate, human kidney tissue, human placenta tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:500
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FC
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1:50-1:100
Target
Function
Cullin 3 is a protein that in humans is encoded by the CUL3 gene. Cullin 3 protein belongs to the family of cullins which in mammals contains eight proteins (Cullin 1, Cullin 2, Cullin 3, Cullin 4A, Cullin 4B, Cullin 5, Cullin 7 and Cullin 9). Cullin proteins are an evolutionarily conserved family of proteins throughout yeast, plants and mammals. Cullin 3 is a component of Cullin-RING E3 ubiquitin ligases complexes (CRLs) which are involved in protein ubiquitylation and represent a part of ubiquitin–proteasome system (UPS). Added ubiquitin moieties to the lysine residue by CRLs then target the protein for the proteasomal degradation. Cullin-RING E3 ubiquitin ligases are involved in many cellular processes responsible for cell cycle regulation, stress response, protein trafficking, signal transduction, DNA replication, transcription, protein quality control, circadian clock and development. Deletion of CUL3 gene in mice causes embryonic lethality.
Background References
1. Li X et al. CUL3 (cullin 3)-mediated ubiquitination and degradation of BECN1 (beclin 1) inhibit autophagy and promote tumor progression. Autophagy. 2021 Dec
2. Wu J et al. Cullin-3: Renal and Vascular Mechanisms Regulating Blood Pressure. Curr Hypertens Rep. 2020 Aug
Sequence Similarity
Belongs to the cullin family.
Tissue Specificity
Brain, spermatozoa, and testis (at protein level). Widely expressed.
Post-translational Modification
Neddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
Subcellular Location
Golgi apparatus. Nucleus.
Synonyms
CUL 3 antibody
Cul-3 antibody
cul3 antibody
CUL3_HUMAN antibody
Cullin-3 antibody
Cullin3 antibody
KIAA0617 antibody
PHA2E antibody
Images
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Western blot analysis of Cullin-3 on 293T lysates with Rabbit anti-Cullin-3 antibody (ER1706-70) at 1/1,000 dilution.
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1706-70, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 89 kDa
Observed band size: 89 kDa -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cullin-3 antibody (ER1706-70) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-70) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cullin-3 antibody. Counter stained with hematoxylin.
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Western blot analysis of Cullin-3 on mouse testis tissue lysates with Rabbit anti-Cullin-3 antibody (ER1706-70) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 89 kDa
Observed band size: 80 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-70) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Cullin 3 Recombinant Rabbit Monoclonal Antibody [JE37-29]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
