Cystatin C Recombinant Rabbit Monoclonal Antibody [PSH18-60]
Catalog# HA724013
Cystatin C Recombinant Rabbit Monoclonal Antibody [PSH18-60]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Cystatin C Recombinant Rabbit Monoclonal Antibody [PSH18-60]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Cystatin C aa 1-146.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 16 kDa
Positive Control
MDA-MB-231 cell lysate, Huh7 cell lysate, TT cell lysate, human kidney tissue, human ovary tissue, mouse kidney tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH18-60
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | IP | |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:20,000
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IHC-P
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1:2,000-1:5,000
Target
Function
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy.
Background References
1. Chen DC et al. Advantages, Limitations, and Clinical Considerations in Using Cystatin C to Estimate GFR. Kidney360. 2022 Aug
2. Skidmore M et al. Cystatin C as a Marker of Kidney Function in Children. Biomolecules. 2024 Aug
Subcellular Location
Secreted
Synonyms
Cystatin-C
Cystatin-3
Gamma-trace
Neuroendocrine basic polypeptide
Post-gamma-globulin
CST3
Images
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☑ Relative expression (RE)
Western blot analysis of Cystatin C on different lysates with Rabbit anti-Cystatin C antibody (HA724013) at 1/20,000 dilution.
Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 2: MOLT-4 cell lysate (negative) (20 µg/Lane)
Lane 3: Huh7 cell lysate (20 µg/Lane)
Lane 4: TT cell lysate (20 µg/Lane)
Predicted band size: 16 kDa
Observed band size: 14 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724013) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cystatin C antibody (HA724013) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724013) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-Cystatin C antibody (HA724013) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724013) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Cystatin C antibody (HA724013) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724013) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of Cystatin C on different lysates with Rabbit anti-Cystatin C antibody (HA724013) at 1/20,000 dilution.
Lane 1: Mouse brain tissue lysate (10 µg/Lane)
Lane 2: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 16 kDa
Observed band size: 16/18 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724013) at 1/20,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"