Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC59-01] - BSA and Azide free
Catalog# HA750202
Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC59-01] - BSA and Azide free
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC59-01] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Cytochrome C aa 10-50.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 12 kDa
Positive Control
HeLa cell lysate, C6 cell lysate, mouse brain tissue lysate, mouse kidney tissue lysate, rat brain tissue lysate, rat kidney tissue lysate, MDA-MB-231 cell lysate, Hela, human liver tissue, mouse liver tissue, rat liver tissue, human kidney tissue, mouse kidney tissue.
Conjugation
unconjugated
Clone Number
SC59-01
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
Cytochrome c is a well characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial intermembrane space. More recent studies have identifed cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is translocated from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.
Background References
1. Barneo-Mu oz M et al. Lack of GDAP1 induces neuronal calcium and mitochondrial defects in a knockout mouse model of charcot-marie-tooth neuropathy. PLoS Genet 11:e1005115 (2015).
2. Conde J et al. Identification of novel adipokines in the joint. Differential expression in healthy and osteoarthritis tissues. PLoS One 10:e0123601 (2015).
Sequence Similarity
Belongs to the cytochrome c family.
Post-translational Modification
Binds 1 heme group per subunit.; Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.
Subcellular Location
Mitochondrion intermembrane space.
Synonyms
CYC antibody
CYC_HUMAN antibody
CYCS antibody
Cytochrome c antibody
Cytochrome c somatic antibody
HCS antibody
THC4 antibody
Images
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This data was developed using ET1610-16, the same antibody clone in a different buffer formulation.
Western blot analysis of Cytochrome C on different lysates with Rabbit anti-Cytochrome C antibody (ET1610-16) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: C6 cell lysate (10 µg/Lane)
Lane 3: Mouse brain tissue lysate (20 µg/Lane)
Lane 4: Mouse kidney tissue lysate (20 µg/Lane)
Lane 5: Rat brain tissue lysate (20 µg/Lane)
Lane 6: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 12 kDa
Observed band size: 12 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-16) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1610-16, the same antibody clone in a different buffer formulation.
Western blot analysis of Cytochrome C on different lysates with Rabbit anti-Cytochrome C antibody (ET1610-16) at 1/1,000 dilution.
Lane 1: MDA-MB-231 cell lysate
Lane 2: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 12 kDa
Observed band size: 12 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-16) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using ET1610-16, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Cytochrome C antibody (ET1610-16) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-16) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1610-16, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Cytochrome C antibody (ET1610-16) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-16) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using ET1610-16, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Cytochrome C antibody (ET1610-16) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-16) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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