DCC Recombinant Rabbit Monoclonal Antibody [PSH12-73]
Catalog# HA723484
DCC Recombinant Rabbit Monoclonal Antibody [PSH12-73]
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WB
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IHC-Fr
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
DCC Recombinant Rabbit Monoclonal Antibody [PSH12-73]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human DCC aa 1-1,097.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-Fr
Target Molecular Weight
Predicted band size: 158 kDa
Positive Control
Mouse brain (P0) tissue lysate, Mouse brain (P7) tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH12-73
Reactivity Data
| WB | IHC-Fr | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
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IHC-Fr
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1:500
Target
Function
The DCC (Deleted in Colorectal Carcinoma) gene encodes a single-pass transmembrane receptor that functions as a receptor for netrin, a key axon guidance molecule in the developing nervous system. DCC plays a critical role in mediating axon attraction by binding to netrin ligands, guiding neuronal growth cones during neural development. Additionally, DCC can associate with UNC5 family proteins to trigger axon repulsion, highlighting its dual role in axon guidance. Beyond its role in the nervous system, DCC acts as a dependence receptor. In the absence of netrin ligand, DCC induces apoptosis, suggesting its function as a tumor suppressor. DCC has long been implicated in colorectal cancer, and its loss or epigenetic silencing is frequently observed in various cancers, supporting its role in tumor suppression. Recent research has revived interest in DCC as a potential therapeutic target due to its ligand-dependent tumor suppressor activity.
Background References
1. Miyake S, et al. Point mutations and allelic deletion of tumor suppressor gene DCC in human esophageal squamous cell carcinomas and their relation to metastasis. Cancer Res. 1994 Jun.
2. Keino-Masu K, et al. Deleted in Colorectal Cancer (DCC) encodes a netrin receptor. Cell. 1996 Oct.
Subcellular Location
Membrane.
Synonyms
Colorectal cancer related chromosome sequence 18 antibody
Colorectal cancer suppressor antibody
CRC 18 antibody
CRC18 antibody
CRCR 1 antibody
CRCR1 antibody
DCC antibody
DCC_HUMAN antibody
Deleted in colorectal cancer protein antibody
Deleted in colorectal carcinoma antibody
ExpandImages
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Western blot analysis of DCC on different lysates with Rabbit anti-DCC antibody (HA723484) at 1/5,000 dilution.
Lane 1: Mouse brain (P0) tissue lysate
Lane 2: Mouse liver tissue lysate (negative)
Lane 3: Mouse brain (P7) tissue lysate
Lane 4: Rat brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 158 kDa
Observed band size: 200 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723484) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Application: IHC-Fr
Species: Mouse
Site: E14.5 embryo
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Application: IHC-Fr
Species: Mouse
Site: E14.5 embryo (brain)
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"