The DYKDDDDK peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, DYKDDDDK tag is unlikely to affect the tagged protein’s biochemical properties. DYKDDDDK tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
DYKDDDDK tag Nanoselector Magnetic beads has been specifically designed to bind DYKDDDDK tag. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer. DYKDDDDK tag Nanoselector Magnetic beads thus features high affinity and superior capacity for DYKDDDDK tag fusion proteins while showing negligible non-specific background. DYKDDDDK tag Nanoselector Magnetic beads is compatible not only with physiological buffers but also with high stringency buffers which provides great freedom to adjust the binding and washing conditions to the experimental needs.
Storage Instructions
Shipped at ambient temperature. Upon receipt store at 4℃. Stable for 1 year. Do not freeze.
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