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E-Cadherin Mouse Monoclonal Antibody [A0-G11-2]

Usd: 350

Specification

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Catalog# EM0502

E-Cadherin Mouse Monoclonal Antibody [A0-G11-2]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

E-Cadherin Mouse Monoclonal Antibody [A0-G11-2]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within mouse E-Cadherin aa 350-550.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 97 kDa

Positive Control

SW480 cell lysate, A431 cell lysate, human liver tissue, mouse liver tissue, mouse colon tissue, rat colon tissue.

Conjugation

unconjugated

Clone Number

A0-G11-2

RRID

AB_3068735

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:500-1:1,000

  • IF-Tissue

  • 1:500

Target

Function

E-cadherin (epithelial) is the most well-studied member of the cadherin family. It consists of 5 cadherin repeats (EC1 ~ EC5) in the extracellular domain, one transmembrane domain, and an intracellular domain that binds p120-catenin and beta-catenin. The intracellular domain contains a highly-phosphorylated region vital to beta-catenin binding and, therefore, to E-cadherin function. Loss of E-cadherin function or expression has been implicated in cancer progression and metastasis. E-cadherin downregulation decreases the strength of cellular adhesion within a tissue, resulting in an increase in cellular motility. This in turn may allow cancer cells to cross the basement membrane and invade surrounding tissues. E-cadherin is also used by pathologists to diagnose different kinds of breast cancer.

Background References

1. Eger A, et al. (Mar 2005). "DeltaEF1 is a transcriptional repressor of E-cadherin and regulates epithelial plasticity in breast cancer cells." Oncogene 24 (14): 2375-85.

2. Liu YN, et al. (Dec 2005). "Regulatory mechanisms controlling human E-cadherin gene expression." Oncogene 24 (56): 8277-90.

3. Lombaerts M, et al. (Mar 2006). "E-cadherin transcriptional downregulation by promoter methylation but not mutation is related to epithelial-to-mesenchymal transition in breast cancer cell lines." Br J Cancer 94 (5): 661-71.

Tissue Specificity

Expressed in inner and outer pillar cells of the organ of Corti (at protein level). Non-neural epithelial tissues.

Post-translational Modification

During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 (By similarity). Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm (By similarity). The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway (By similarity). Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system (By similarity). The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions (By similarity). During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation.; O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Ser-287 and Thr-511 are O-manosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3.; N-glycosylation at Asn-639 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location (By similarity).; Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-756 (By similarity).

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P12830 Human

SwissProt: P09803 Mouse

SwissProt: Q9R0T4 Rat

Synonyms

Arc 1 antibody

CADH1_HUMAN antibody

Cadherin 1 antibody

cadherin 1 type 1 E-cadherin antibody

Cadherin1 antibody

CAM 120/80 antibody

CD 324 antibody

CD324 antibody

CD324 antigen antibody

cdh1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of E-Cadherin with anti-E-Cadherin antibody [A0-G11-2] (<a href="/products/EM0502" style="font-weight: bold;text-decoration: underline;">EM0502</a>) at 1:1,000 dilution.<br />Lane 1/2: Wild-type A431 whole cell lysate (20 µg).<br />Lane 3/4: E-Cadherin fragment 1 knockdown A431 whole cell lysate (20 µg).<br />Lane 5/6: E-Cadherin fragment 2 knockdown A431 whole cell lysate (20 µg).<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM0502" style="font-weight: bold;text-decoration: underline;">EM0502</a>, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , <a href="/products/ET1601-4" style="font-weight: bold;text-decoration: underline;">ET1601-4</a>, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/20,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of E-Cadherin with anti-E-Cadherin antibody [A0-G11-2] (EM0502) at 1:1,000 dilution.
    Lane 1/2: Wild-type A431 whole cell lysate (20 µg).
    Lane 3/4: E-Cadherin fragment 1 knockdown A431 whole cell lysate (20 µg).
    Lane 5/6: E-Cadherin fragment 2 knockdown A431 whole cell lysate (20 µg).

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (EM0502, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody (HA1006) at 1/20,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (<a href="/products/EM0502" style="font-weight: bold;text-decoration: underline;">EM0502</a>) at 1/500 dilution.<br /><br />Lane 1: SW480 cell lysate<br />Lane 2: A431 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 98 kDa<br />Observed band size: 130 kDa<br /><br />Exposure time: 2 minutes;<br />6% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM0502" style="font-weight: bold;text-decoration: underline;">EM0502</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (EM0502) at 1/500 dilution.

    Lane 1: SW480 cell lysate
    Lane 2: A431 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 98 kDa
    Observed band size: 130 kDa

    Exposure time: 2 minutes;
    6% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM0502) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-E-Cadherin antibody (<a href="/products/EM0502" style="font-weight: bold;text-decoration: underline;">EM0502</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM0502" style="font-weight: bold;text-decoration: underline;">EM0502</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-E-Cadherin antibody (EM0502) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0502) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-E-Cadherin antibody (<a href="/products/EM0502" style="font-weight: bold;text-decoration: underline;">EM0502</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM0502" style="font-weight: bold;text-decoration: underline;">EM0502</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-E-Cadherin antibody (EM0502) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0502) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-E-Cadherin antibody (<a href="/products/EM0502" style="font-weight: bold;text-decoration: underline;">EM0502</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM0502" style="font-weight: bold;text-decoration: underline;">EM0502</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-E-Cadherin antibody (EM0502) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0502) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-E-Cadherin antibody (<a href="/products/EM0502" style="font-weight: bold;text-decoration: underline;">EM0502</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM0502" style="font-weight: bold;text-decoration: underline;">EM0502</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-E-Cadherin antibody (EM0502) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0502) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: colon<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Mouse

    Site: colon

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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