E-Cadherin Recombinant Mouse Monoclonal Antibody
Catalog# IRS031
E-Cadherin Recombinant Mouse Monoclonal Antibody
-
mIHC
-
Human
-
unconjugated
Overview
Product Name
E-Cadherin Recombinant Mouse Monoclonal Antibody
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Recombinant protein within mouse E-Cadherin aa 350-550.
Species Reactivity
Human
Validated Applications
mIHC
Molecular Weight
Predicted band size: 98 kDa
Positive Control
Human pancreas tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
-
1:100
Target
Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
Background References
1. Thomas E Meigs et al. Galpha12 and Galpha13 negatively regulate the adhesive functions of cadherin. J Biol Chem 277(27):24594-600 (2002)
2. Georgia Agiostratidou et al. The cytoplasmic sequence of E-cadherin promotes non-amyloidogenic degradation of A beta precursors. 96(4):1182-8 (2006)
Subcellular Location
Cell membrane, Endosome, Golgi apparatus.
UNIPROT
Synonyms
Cadherin-1
CAM 120/80
Epithelial cadherin (E-cadherin)
Uvomorulin
Images
-
mIHC analysis of human pancreas tissue (Formalin/PFA-fixed paraffin-embedded sections) with Mouse anti-E-Cadherin antibody (IRS031) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900809). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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