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MTERFD1 Mouse Monoclonal Antibody [A8-A10]

Usd: 350

Specification

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Catalog# EM1701-29

MTERFD1 Mouse Monoclonal Antibody [A8-A10]

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

MTERFD1 Mouse Monoclonal Antibody [A8-A10]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein with Human MTERFD1 aa 29-417 / 417.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Cell

Molecular Weight

Predicted band size: 48 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, K-562 cell lysate, HEK-293 cell lysate, SH-SY5Y cell lysate, SK-OV-3 cell lysate, human liver tissue, A431.

Conjugation

unconjugated

Clone Number

A8-A10

RRID

AB_3068754

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

  • IF-Cell

  • 1:50

Target

Function

MTERFD1 belongs to the MTERF family which consists of four members including MTERF1, MTERF2, MTERF3 and MTERF4. Previous research showed that MTERFD1 is a mitochondrial protein that interacts with the mitochondrial DNA promoter region and decreases transcription initiation in mammalian mitochondria. This negative regulation is likely important for fine-tuning mitochondria transcription in response to physiological demands. Human mitochondrial transcription termination factor domain 1 (MTERFD1) was mutated in many types of cancer. In vivo and in vitro data indicated that MTERFD1 gene possesses oncogenic properties.

Background References

暂无

Sequence Similarity

Belongs to the mTERF family.

Tissue Specificity

Highly expressed in heart, liver, kidney and testis. Detected at lower levels in brain, spleen and lung.

Subcellular Location

Mitochondrion.

UNIPROT

SwissProt: Q96E29 Human

SwissProt: Q8R3J4 Mouse

Synonyms

CGI 12 antibody

mitochondrial antibody

Mitochondrial transcription termination factor 3 antibody

MTER1_HUMAN antibody

MTERF domain containing 1 antibody

mTERF domain containing protein 1 mitochondrial antibody

mTERF domain-containing protein 1 antibody

mTERF3 antibody

Mterfd1 antibody

Transcription termination factor 3, mitochondrial antibody

Images

  • Western blot analysis of MTERFD1 on different lysates with Mouse anti-MTERFD1 antibody (<a href="/products/EM1701-29" style="font-weight: bold;text-decoration: underline;">EM1701-29</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: K-562 cell lysate<br />Lane 4: HEK-293 cell lysate<br />Lane 5: SH-SY5Y cell lysate<br />Lane 6: SK-OV-3 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 48 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-29" style="font-weight: bold;text-decoration: underline;">EM1701-29</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MTERFD1 on different lysates with Mouse anti-MTERFD1 antibody (EM1701-29) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: K-562 cell lysate
    Lane 4: HEK-293 cell lysate
    Lane 5: SH-SY5Y cell lysate
    Lane 6: SK-OV-3 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 48 kDa
    Observed band size: 40 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-29) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MTERFD1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MTERFD1 antibody. Counter stained with hematoxylin.

  • Immunocytochemistry analysis of A431 cells labeling MTERFD1 with Mouse anti-MTERFD1 antibody (<a href="/products/EM1701-29" style="font-weight: bold;text-decoration: underline;">EM1701-29</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-MTERFD1 antibody (<a href="/products/EM1701-29" style="font-weight: bold;text-decoration: underline;">EM1701-29</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of A431 cells labeling MTERFD1 with Mouse anti-MTERFD1 antibody (EM1701-29) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-MTERFD1 antibody (EM1701-29) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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MTERFD1 Recombinant Mouse Monoclonal Antibody [A8-A10-R]

Application: WB

Reactivity: Human,Mouse

Conjugate: unconjugated