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PTBP1 Mouse Monoclonal Antibody [9A1]

Usd: 350

Specification

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Catalog# EM1701-63

PTBP1 Mouse Monoclonal Antibody [9A1]

Application

  • WB

  • IHC-P

  • IF-Cell

  • FC

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

PTBP1 Mouse Monoclonal Antibody [9A1]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within human PTBP1 aa 320-550.

Species Reactivity

Human, Rat

Validated Applications

WB, IHC-P, IF-Cell, FC

Molecular Weight

Predicted band size: 57 kDa

Positive Control

Jurkat cell lysate, A431 cell lysate, K562 cell lysate, A549, 293T, SW480, human kidney tissue, human tonsil tissue, rat testis tissue, human colon cancer tissue, K562.

Conjugation

unconjugated

Clone Number

9A1

RRID

AB_3068787

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG2a

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:5,000-1:10,000

  • IHC-P

  • 1:50-1:100

  • IF-Cell

  • 1:50-1:100

  • FC

  • 1:50-1:100

Target

Function

Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation.

Background References

1. Zhu W et al. Roles of PTBP1 in alternative splicing, glycolysis, and oncogensis. J Zhejiang Univ Sci B. 2020 Feb

2. Huan L et al. Hypoxia induced LUCAT1/PTBP1 axis modulates cancer cell viability and chemotherapy response. Mol Cancer. 2020 Jan

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P26599 Human

SwissProt: Q00438 Rat

Synonyms

57 kDa RNA binding protein PPTB 1 antibody

57 kDa RNA-binding protein PPTB-1 antibody

Heterogeneous nuclear ribonucleoprotein I antibody

Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody

hnRNP I antibody

HNRNP-I antibody

HNRNPI antibody

HNRPI antibody

MGC10830 antibody

MGC8461 antibody

Expand

Images

  • Western blot analysis of PTBP1 on different lysates with Mouse anti-PTBP1 antibody (<a href="/products/EM1701-63" style="font-weight: bold;text-decoration: underline;">EM1701-63</a>) at 1/5,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: A431 cell lysate<br />Lane 3: K562 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 57 kDa<br />Observed band size: 57 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1701-63" style="font-weight: bold;text-decoration: underline;">EM1701-63</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PTBP1 on different lysates with Mouse anti-PTBP1 antibody (EM1701-63) at 1/5,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: A431 cell lysate
    Lane 3: K562 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-63) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

  • ICC staining PTBP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining PTBP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining PTBP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining PTBP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining PTBP1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining PTBP1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"