Glucocorticoid Receptor alpha Mouse Monoclonal Antibody [3F1]
Catalog# EM1701-65
Glucocorticoid Receptor alpha Mouse Monoclonal Antibody [3F1]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
Glucocorticoid Receptor alpha Mouse Monoclonal Antibody [3F1]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant full length protein corresponding to Human Glucocorticoid Receptor alpha.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
Predicted band size: 86 kDa
Positive Control
SiHa cell lysate, A549 cell lysate, Hela, human spleen tissue, human breast carcinoma tissue, A549.
Conjugation
unconjugated
Clone Number
3F1
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2a
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that mediates the effects of glucocorticoids. The most abundant isoform is GR α. GR induces or represses the expression of genes in response to glucocorticoids, mediating such processes as apoptosis, cell growth and differentiation. A significant class of genes suppressed by GR is controlled by the transcription factor AP-1. GR has also been shown to be the limiting factor in the induction of gene expression by glucocorticoids. It has been revealed that GR forms a complex with HSP 90, rendering the non-ligand bound receptor transcriptionally inactive. More importantly, mutant GRs lacking the signaling domain remain constitutively active.
Background References
1. Vassiliou AG et al. Increased Glucocorticoid Receptor Alpha Expression and Signaling in Critically Ill Coronavirus Disease 2019 Patients. Crit Care Med. 2021 Dec
2. Meduri GU et al. General Adaptation in Critical Illness: Glucocorticoid Receptor-alpha Master Regulator of Homeostatic Corrections. Front Endocrinol (Lausanne). 2020 Apr
Sequence Similarity
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Tissue Specificity
Widely expressed including bone, stomach, lung, liver, colon, breast, ovary, pancreas and kidney. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Isoform Beta: Widely expressed including brain, bone marrow, thymus, spleen, liver, kidney, pancreas, lung, fat, skeletal muscle, heart, placenta and blood leukocytes. Isoform Alpha-2: Expressed at low level.
Post-translational Modification
Acetylation by CLOCK reduces its binding to glucocorticoid response elements and its transcriptional activity.; Increased proteasome-mediated degradation in response to glucocorticoids. Isoform Alpha-B appears to be more susceptible to proteolytic degradation than isoform Alpha.; Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203, Ser-226 and Ser-404-phosphorylated forms are mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Phosphorylation at Ser-211 increases transcriptional activity. Phosphorylation at Ser-203, Ser-226 and Ser-404 decreases signaling capacity. Phosphorylation at Ser-404 may protect from glucocorticoid-induced apoptosis. Phosphorylation at Ser-203 and Ser-211 is not required in regulation of chromosome segregation. May be dephosphorylated by PPP5C, attenuates NR3C1 action (By similarity).; Sumoylation at Lys-277 and Lys-293 negatively regulates its transcriptional activity. Sumoylation at Lys-703 positively regulates its transcriptional activity in the presence of RWDD3 (By similarity). Sumoylation at Lys-277 and Lys-293 is dispensable whereas sumoylation at Lys-703 is critical for the stimulatory effect of RWDD3 on its transcriptional activity (By similarity). Heat shock increases sumoylation in a RWDD3-dependent manner (By similarity).; Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
Subcellular Location
Nucleus. Cytoskeleton. Mitochondrion.
UNIPROT
Synonyms
GCCR antibody
GCR antibody
Glucocorticoid receptor alpha isoform antibody
Glucocorticoid receptor antibody
GR antibody
GRL antibody
NR3C1 antibody
Nuclear receptor subfamily 3 group C member 1 antibody
Images
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Western blot analysis of Glucocorticoid Receptor alpha on different lysates with Mouse anti-Glucocorticoid Receptor alpha antibody (EM1701-65) at 1/5,000 dilution.
Lane 1: SiHa cell lysate
Lane 2: A549 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 86 kDa
Observed band size: 100 kDa
Exposure time: 5 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-65) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Hela cells labeling Glucocorticoid Receptor alpha with Mouse anti-Glucocorticoid Receptor alpha antibody (EM1701-65) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Glucocorticoid Receptor alpha antibody (EM1701-65) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Glucocorticoid Receptor alpha antibody (EM1701-65) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-65) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"