ALDH4A1 Mouse Monoclonal Antibody [7E2]
Catalog# EM1701-74
ALDH4A1 Mouse Monoclonal Antibody [7E2]
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WB
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IHC-P
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IF-Cell
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IF-Tissue
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ALDH4A1 Mouse Monoclonal Antibody [7E2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within Human ALDH4A1 aa 514-563 / 563.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell, IF-Tissue
Molecular Weight
Predicted band size: 62 kDa
Positive Control
HepG2 cell lysate, SK-Br-3 cell lysate, human kidney tissue lysate, human liver tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, HepG2, human kidney tissue, human liver tissue, mouse liver tissue, rat liver tissue.
Conjugation
unconjugated
Clone Number
7E2
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IHC-P
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1:1,000
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IF-Cell
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1:100
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IF-Tissue
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1:100
Target
Function
Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde, lipid peroxidation and metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH4A1 (aldehyde dehydrogenase 4 family member A1), also known as P5CD (Delta-1-pyrroline-5-carboxylate dehydrogenase), P5CDh, P5CDhL, P5CDhS or ALDH4, is a major enzyme involved in the proline degradation pathway. Localizing to the mitochondrial matrix, ALDH4A1 catalyzes the conversion of Delta-1-pyrroline-5-carboxylate (P5C) to glutamate. A mutation in the gene encoding ALDH4A1 results in HPII (hyperprolinemia type II), a disease characterized by an excess of P5C and proline that is associated with mental retardation and seizures.
Background References
1. Srivastava D et al. The three-dimensional structural basis of type II hyperprolinemia. J Mol Biol 420:176-189 (2012).
2. Hochstrasser D F et al. Human liver protein map: a reference database established by microsequencing and gel comparison. Electrophoresis 13:992-1001 (1992).
Sequence Similarity
Belongs to the aldehyde dehydrogenase family.
Tissue Specificity
Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas.
Subcellular Location
Mitochondrion matrix.
Synonyms
AL4A1_HUMAN antibody
aldehyde dehydrogenase 4 antibody
aldehyde dehydrogenase 4 family, member A1 antibody
Aldehyde dehydrogenase family 4 member A1 antibody
Aldehyde dehydrogenase, family 4, subfamily A, member 1 antibody
ALDH4 antibody
aldh4a1 antibody
Delta 1 pyrroline 5 carboxylate dehydrogenase, mitochondrial antibody
Delta-1-pyrroline-5-carboxylate dehydrogenase antibody
L-glutamate gamma-semialdehyde dehydrogenase antibody
ExpandImages
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Western blot analysis of ALDH4A1 on different lysates with Mouse anti-ALDH4A1 antibody (EM1701-74) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: SK-Br-3 cell lysate (20 µg/Lane)
Lane 3: Human kidney tissue lysate (40 µg/Lane)
Lane 4: Human liver tissue lysate (40 µg/Lane)
Lane 5: Mouse liver tissue lysate (40 µg/Lane)
Lane 6: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 62 kDa
Observed band size: 55 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-74) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HepG2 cells labeling ALDH4A1 with Mouse anti-ALDH4A1 antibody (EM1701-74) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH4A1 antibody (EM1701-74) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-ALDH4A1 antibody (EM1701-74) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ALDH4A1 antibody (EM1701-74) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ALDH4A1 antibody (EM1701-74) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-ALDH4A1 antibody (EM1701-74) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded mouse liver tissue labeling ALDH4A1 with Mouse anti-ALDH4A1 antibody (EM1701-74) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM1701-74, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"