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PAX8 Mouse Monoclonal Antibody [6G5]

Usd: 350

Specification

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Catalog# EM1701-81

PAX8 Mouse Monoclonal Antibody [6G5]

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

PAX8 Mouse Monoclonal Antibody [6G5]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide corresponding to human PAX8 aa 400-450.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Cell

Molecular Weight

Predicted band size: 48 kDa

Positive Control

Skov-3 cells lysates, NIH:OVCAR-3, human kidney tissue, human thyroid tissue, human thyroid cancer tissue, mouse thyroid tissue.

Conjugation

unconjugated

Clone Number

6G5

RRID

AB_3068805

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:2,000

  • IF-Cell

  • 1:100

Target

Function

The PAX gene family has an important role in the formation of tissues and organs during embryonic development and maintaining the normal function of some cells after birth. The PAX genes give instructions for making proteins that attach themselves to certain areas of DNA. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. PAX8 releases the hormones important for regulating growth, brain development, and metabolism. Also functions in very early stages of kidney organogenesis, the müllerian system, and the thymus. Additionally, PAX8 is expressed in the renal excretory system, epithelial cells of the endocervix, endometrium, ovary, Fallopian tube, seminal vesicle, epididymis, pancreatic islet cells and lymphoid cells. PAX8 and other transcription factors play a role in binding to DNA and regulating the genes that drive thyroid hormone synthesis (Tg, TPO, Slc5a5 and Tshr). This PAX-8 antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalinfixed, paraffin-embedded tissue sections using IHC test methods.

Background References

1. Laury AR et al. A comprehensive analysis of PAX8 expression in human epithelial tumors. The American Journal of Surgical Pathology 35 (6): 816–26 (2011).

2. Fernández LP et al. Thyroid transcription factors in development, differentiation and disease. Nature Reviews Endocrinology 11 (1): 29-42 (2015).

Tissue Specificity

Expressed in the excretory system, thyroid gland and Wilms tumors.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q06710 Human

SwissProt: Q00288 Mouse

Synonyms

OTTHUMP00000158659 antibody

OTTHUMP00000158660 antibody

OTTHUMP00000203723 antibody

OTTHUMP00000203724 antibody

Paired box 8 antibody

Paired box gene 8 antibody

paired box homeotic gene 8 antibody

Paired box protein Pax 8 antibody

Paired box protein Pax-8 antibody

Paired domain gene 8 antibody

Expand

Images

  • Western blot analysis of Pax8 on skov-3 cells lysates using anti-Pax8 antibody at 1/500 dilution.

    Western blot analysis of Pax8 on skov-3 cells lysates using anti-Pax8 antibody at 1/500 dilution.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunocytochemistry analysis of NIH:OVCAR-3 (positive) and HeLa (negative) labeling PAX8 with Mouse anti-PAX8 antibody (<a href="/products/EM1701-81" style="font-weight: bold;text-decoration: underline;">EM1701-81</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PAX8 antibody (<a href="/products/EM1701-81" style="font-weight: bold;text-decoration: underline;">EM1701-81</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    ☑ Relative expression (RE)

    Immunocytochemistry analysis of NIH:OVCAR-3 (positive) and HeLa (negative) labeling PAX8 with Mouse anti-PAX8 antibody (EM1701-81) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PAX8 antibody (EM1701-81) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-PAX8 antibody (<a href="/products/EM1701-81" style="font-weight: bold;text-decoration: underline;">EM1701-81</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1701-81" style="font-weight: bold;text-decoration: underline;">EM1701-81</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-PAX8 antibody (EM1701-81) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-81) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Mouse anti-PAX8 antibody (<a href="/products/EM1701-81" style="font-weight: bold;text-decoration: underline;">EM1701-81</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1701-81" style="font-weight: bold;text-decoration: underline;">EM1701-81</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Mouse anti-PAX8 antibody (EM1701-81) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-81) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Mouse anti-PAX8 antibody (<a href="/products/EM1701-81" style="font-weight: bold;text-decoration: underline;">EM1701-81</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1701-81" style="font-weight: bold;text-decoration: underline;">EM1701-81</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Mouse anti-PAX8 antibody (EM1701-81) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-81) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue with Mouse anti-PAX8 antibody (<a href="/products/EM1701-81" style="font-weight: bold;text-decoration: underline;">EM1701-81</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1701-81" style="font-weight: bold;text-decoration: underline;">EM1701-81</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue with Mouse anti-PAX8 antibody (EM1701-81) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-81) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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