BTN2A2 Mouse Monoclonal Antibody [4B11C8]
Catalog# EM1710-73
BTN2A2 Mouse Monoclonal Antibody [4B11C8]
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WB
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IF-Cell
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FC
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Human
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unconjugated
Overview
Product Name
BTN2A2 Mouse Monoclonal Antibody [4B11C8]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Purified recombinant fragment of human BTN2A2 (AA: extra 57-237) expressed in E. Coli.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Molecular Weight
59kDa
Positive Control
K562 cell lysate, Hela cells
Conjugation
unconjugated
Clone Number
4B11C8
Product Features
Form
Liquid
Concentration
Storage Instructions
4℃; -20℃ for long term storage.
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide.
Isotype
IGg2a
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-cell
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1：50-1:200
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FC
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1:100-1:200
Target
Function
Butyrophilin is the major protein associated with fat droplets in the milk. This gene is a member of the BTN2 subfamily of genes, which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6, consisting of seven genes belonging to the expanding B7/butyrophilin-like group, a subset of the immunoglobulin gene superfamily. The encoded protein is a type I receptor glycoprotein involved in lipid, fatty-acid and sterol metabolism. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background References
1. J Exp Med. 2016 Feb 8;213(2):177-87.
2. Proteomics. 2002 Jul;2(7):850-6.
Subcellular Location
Membrane.
UNIPROT
Synonyms
BT2.2 antibody
BT2A2_HUMAN antibody
BTF2 antibody
BTN2A2 antibody
butyrophilin 2 antibody
Butyrophilin subfamily 2 member A2 antibody
Images
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Western blot analysis of BTN2A2 against human BTN2A2 (AA: extra 57-237) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
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Western blot analysis of BTN2A2 against HEK293 (1) and BTN2A2 (AA: extra 57-237)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
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Western blot analysis of BTN2A2 against K562 (1) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1710-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunocytochemistry staining of BTN2A2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1710-73, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue), Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
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Flow cytometric analysis of BTN2A2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1710-73, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"