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CD307C Mouse Monoclonal Antibody [4B4B2]

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Catalog# EM1711-77

CD307C Mouse Monoclonal Antibody [4B4B2]

Application

  • WB

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

CD307C Mouse Monoclonal Antibody [4B4B2]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Purified recombinant fragment of human CD307C (AA: extra 18-153) expressed in E. Coli.

Species Reactivity

Human

Validated Applications

WB, FC

Molecular Weight

80.9kDa

Positive Control

HL-60 cells

Conjugation

unconjugated

Clone Number

4B4B2

Product Features

Form

Liquid

Concentration

Storage Instructions

4℃; -20℃ for long term storage.

Storage Buffer

Purified antibody in PBS with 0.05% sodium azide.

Isotype

IgG2a

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • FC

  • 1:100-1:200

Target

Function

This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains immunoreceptor-tyrosine activation motifs and immunoreceptor-tyrosine inhibitory motifs in its cytoplasmic domain and may play a role in regulation of the immune system. Mutations in this gene have been associated with rheumatoid arthritis, autoimmune thyroid disease, and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.

Background References

1. Hum Immunol. 2016 Feb;77(2):223-9.

2. Autoimmunity. 2013 Dec;46(8):547-58.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q96P31 Human

Synonyms

CD307c antibody

Fc receptor homolog 3 antibody

Fc receptor-like protein 3 antibody

FcR-like protein 3 antibody

FcRH3 antibody

FcRL3 antibody

FCRL3_HUMAN antibody

hIFGP3 antibody

IFGP family protein 3 antibody

IFGP3 antibody

Expand

Images

  • Western blot analysis of CD307C against human CD307C (AA: extra 18-153) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/EM1711-77" style="font-weight: bold;text-decoration: underline;">EM1711-77</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD307C against human CD307C (AA: extra 18-153) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of CD307C against HEK293 (1) and CD307C (AA: extra 18-153)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/EM1711-77" style="font-weight: bold;text-decoration: underline;">EM1711-77</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD307C against HEK293 (1) and CD307C (AA: extra 18-153)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1711-77, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of CD307C was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/EM1711-77" style="font-weight: bold;text-decoration: underline;">EM1711-77</a>, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

    Flow cytometric analysis of CD307C was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (EM1711-77, 1/100) (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

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