CD215 Mouse Monoclonal Antibody [5F10D9]
Catalog# EM1712-22
CD215 Mouse Monoclonal Antibody [5F10D9]
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WB
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IF-Cell
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Human
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unconjugated
Overview
Product Name
CD215 Mouse Monoclonal Antibody [5F10D9]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Purified recombinant fragment of human CD215 (AA: extra 31-205) expressed in E. Coli.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Molecular Weight
28.2kDa
Positive Control
LOVO and HCT116 cell lysate
Conjugation
unconjugated
Clone Number
5F10D9
Product Features
Form
Liquid
Concentration
Storage Instructions
4℃; -20℃ for long term storage.
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-cell
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1:50-1:200
Target
Function
This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.
Background References
1. J Cancer Res Ther. 2018 Sep;14(Supplement):S648-S655.
2. BMC Cancer. 2017 Sep 11;17(1):640.
Subcellular Location
Secreted. Nucleus membrane. Golgi apparatus membrane. Cytoplasmic vesicle membrane.
UNIPROT
Synonyms
AA690181 antibody
CD215 antibody
I15RA_HUMAN antibody
IL 15R alpha antibody
IL-15 receptor subunit alpha antibody
IL-15R-alpha antibody
IL-15RA antibody
Il15ra antibody
Interleukin 15 receptor alpha antibody
Interleukin 15 receptor subunit alpha antibody
ExpandImages
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Western blot analysis of CD215 against human CD215 (AA: extra 31-205) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-22, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
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Western blot analysis of CD215 against HEK293 (1) and CD215 (AA: extra 31-205)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-22, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
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Western blot analysis of CD215 against LOVO (1) and HCT116 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-22, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"