IMP3 Mouse Monoclonal Antibody [4-C2-5]
Catalog# EM1801-01
IMP3 Mouse Monoclonal Antibody [4-C2-5]
-
WB
-
FC
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
IMP3 Mouse Monoclonal Antibody [4-C2-5]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human IMP3 aa 350-579.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 64 kDa
Positive Control
HeLa cell lysate, MDA-MB-231 cell lysate, 293T cell lysate, Mouse spleen tissue lysate, Mouse placenta tissue lysate, Rat spleen tissue lysate, Rat placenta tissue lysate, PANC-1.
Conjugation
unconjugated
Clone Number
4-C2-5
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
-
1:2,000
-
FC
-
1:50-1:100
Target
Function
RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.
Background References
1. Vikesaa J et al. RNA-binding IMPs promote cell adhesion and invadopodia formation. EMBO J. 25:1456-1468(2006).
2. Wachter K et al. Subcellular localization and RNP formation of IGF2BPs (IGF2 mRNA-binding proteins) is modulated by distinct RNA-binding domains. Biol Chem 394:1077-1090(2013).
Sequence Similarity
Belongs to the RRM IMP/VICKZ family.
Tissue Specificity
Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.
Subcellular Location
Nucleus.
Synonyms
Cancer/testis antigen 98 antibody
CT98 antibody
DKFZp686F1078 antibody
hKOC antibody
IF2B3_HUMAN antibody
IGF II mRNA binding protein 3 antibody
IGF-II mRNA-binding protein 3 antibody
IGF2 mRNA binding protein 3 antibody
IGF2 mRNA-binding protein 3 antibody
IGF2BP3 antibody
ExpandImages
-
Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1801-01) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: Mouse spleen tissue lysate (40 µg/Lane)
Lane 5: Mouse placenta tissue lysate (40 µg/Lane)
Lane 6: Rat spleen tissue lysate (40 µg/Lane)
Lane 7: Rat placenta tissue lysate (40 µg/Lane)
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: Lane 1-4: 20 seconds; Lane 5-6: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-01) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1801-01) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-IMP3 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 64 kDa
Observed band size: 64 kDa
Exposure time: 12 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-01) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"