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Cytokeratin 5+6 Mouse Monoclonal Antibody [A2B2]

Usd: 350

Specification

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Catalog# EM1901-08

Cytokeratin 5+6 Mouse Monoclonal Antibody [A2B2]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Cytokeratin 5+6 Mouse Monoclonal Antibody [A2B2]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within Human Cytokeratin 5 aa 340-380.

Species Reactivity

Human, Rat

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 60/62 kDa

Positive Control

HT-29 cell lysate, A549 cell lysate, MCF7 cell lysate, HeLa cell lysate, human tonsil tissue, human skin tissue, human lung adenocarcinoma tissue, rat skin tissue, A431.

Conjugation

unconjugated

Clone Number

A2B2

RRID

AB_3068853

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:200-1:1,000

  • FC

  • 1:50-1:100

Target

Function

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Background References

暂无

Sequence Similarity

Belongs to the intermediate filament family.

Tissue Specificity

Expressed in the corneal epithelium (at protein level).

Subcellular Location

Cytoskeleton.

UNIPROT

SwissProt: P02538 Human

SwissProt: P04259 Human

SwissProt: P13647 Human

SwissProt: Q6P6Q2 Rat

SwissProt: Q4FZU2 Rat

Synonyms

58 kDa cytokeratin antibody

CK-5 antibody

CK5 antibody

Cytokeratin-5 antibody

Cytokeratin5 antibody

DDD antibody

DDD1 antibody

EBS2 antibody

epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody

K2C5_HUMAN antibody

Expand

Images

  • Western blot analysis of Cytokeratin 5+6 on different lysates with Mouse anti-Cytokeratin 5+6 antibody (<a href="/products/EM1901-08" style="font-weight: bold;text-decoration: underline;">EM1901-08</a>) at 1/1,000 dilution.<br /><br />Lane 1: HT-29 cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: MCF7 cell lysate<br />Lane 4: HeLa cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 60/62 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM1901-08" style="font-weight: bold;text-decoration: underline;">EM1901-08</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cytokeratin 5+6 on different lysates with Mouse anti-Cytokeratin 5+6 antibody (EM1901-08) at 1/1,000 dilution.

    Lane 1: HT-29 cell lysate
    Lane 2: A549 cell lysate
    Lane 3: MCF7 cell lysate
    Lane 4: HeLa cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 60/62 kDa
    Observed band size: 50 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-08) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 5+6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-08" style="font-weight: bold;text-decoration: underline;">EM1901-08</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 5+6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-08, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-Cytokeratin 5+6 antibody (<a href="/products/EM1901-08" style="font-weight: bold;text-decoration: underline;">EM1901-08</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-08" style="font-weight: bold;text-decoration: underline;">EM1901-08</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-Cytokeratin 5+6 antibody (EM1901-08) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-08) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Mouse anti-Cytokeratin 5+6 antibody (<a href="/products/EM1901-08" style="font-weight: bold;text-decoration: underline;">EM1901-08</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-08" style="font-weight: bold;text-decoration: underline;">EM1901-08</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Mouse anti-Cytokeratin 5+6 antibody (EM1901-08) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-08) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat skin tissue with Mouse anti-Cytokeratin 5+6 antibody (<a href="/products/EM1901-08" style="font-weight: bold;text-decoration: underline;">EM1901-08</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM1901-08" style="font-weight: bold;text-decoration: underline;">EM1901-08</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat skin tissue with Mouse anti-Cytokeratin 5+6 antibody (EM1901-08) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-08) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of Cytokeratin 5+6 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/EM1901-08" style="font-weight: bold;text-decoration: underline;">EM1901-08</a>, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Cytokeratin 5+6 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-08, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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