Aspartate Aminotransferase Mouse Monoclonal Antibody [A2D6]
Catalog# EM1901-51
Aspartate Aminotransferase Mouse Monoclonal Antibody [A2D6]
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WB
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Human
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unconjugated
Overview
Product Name
Aspartate Aminotransferase Mouse Monoclonal Antibody [A2D6]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human GOT1 aa 16-211 / 413.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 46 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate, HL-60 cell lysate, Caco-2 cell lysate, human liver tissue lysate, mouse liver tissue lysate, mouse heart tissue lysate, rat liver tissue lysate, rat heart tissue lysate.
Conjugation
unconjugated
Clone Number
A2D6
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG2b
Purification Method
Protein G affinity purified.
Application Dilution
-
WB
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1:500-1:2,000
Target
Function
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
Background References
1. Abrego J. et. al. GOT1-mediated anaplerotic glutamine metabolism regulates chronic acidosis stress in pancreatic cancer cells. Cancer Lett. 2017 Aug 1;400:37-46.
2. Hong C. et. al. Inhibition of GOT1 sensitizes colorectal cancer cells to 5-fluorouracil. Cancer Chemother Pharmacol. 2017 Apr;79(4):835-840.
Sequence Similarity
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Subcellular Location
Cytoplasm.
Synonyms
AATC_HUMAN antibody
Aspartate aminotransferase 1 antibody
Aspartate aminotransferase antibody
Aspartate aminotransferase cytoplasmic antibody
Aspartate aminotransferase cytosolic antibody
ASTQTL1 antibody
cAspAT antibody
cCAT antibody
cysteine aminotransferase, cytoplasmic antibody
cysteine transaminase, cytoplasmic antibody
ExpandImages
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Western blot analysis of Aspartate Aminotransferase on different lysates with Mouse anti-Aspartate Aminotransferase antibody (EM1901-51) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: HL-60 cell lysate (20 µg/Lane)
Lane 4: Caco-2 cell lysate (20 µg/Lane)
Lane 5: Human liver tissue lysate (40 µg/Lane)
Lane 6: Mouse liver tissue lysate (40 µg/Lane)
Lane 7: Mouse heart tissue lysate (40 µg/Lane)
Lane 8: Rat liver tissue lysate (40 µg/Lane)
Lane 9: Rat heart tissue lysate (40 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 38 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-51) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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